In vivo Impact of Monocrotophos on Biochemical Parameters of a Freshwater Fish during Subacute Toxicity and Following Cessation of Exposure to the Insecticide

Abstract

In vivo toxicity of monocrotophos on key metabolites and enzymes of the protein metabolism was investigated in important tissues of the freshwater fi sh Clarias batrachus. Fish were exposed to 1/10 and 1/20 of LC 50 concentration for 28 days. After 28 days of exposure, some fi sh were transferred to monocrotophos-free water and kept in the same for 21 days (recovery period) in order to study the recovery response. Total protein, amino acid, and ammonia contents were decreased in gill, kidney, liver, and muscle tissues, and recovery was slight at the end of 21 days of transfer of fi sh into freshwater. Urea and glutamine levels were elevated, except in kidneys, and recovered at the end of the recovery period. The activities of protease, transaminase, and phosphatase enzymes were elevated in all tissues during 28 days of exposure and at both concentrations. Recovery of the activity of enzymes was more signifi cant at the lower concentration as compared to the higher concentration.

Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles

“…The experiment was carried out in triplicate of four different sets with the first three groups (A, B and C) without ZnO-NPs but each was only fed with basal diet, 50 mg.kg -1 AA (lowest AA concentration) and 1000 mg.kg -1 AA (highest AA) supplemented diet, respectively. The remaining five groups (D, E, F, G and H) were exposed to 1/10 of the 96 h LC 50 value (Narra et al, 2011) estimated to be 10 mg/l. The test solution (1/10 of the 96 h LC 50 of ZnO-NPs) was renewed on daily basis to maintain the concentration of the toxicant.…”