Enzymatic Biotesting: Scientific Basis and Application

Esimbekova, Elena N.; Torgashina, I. G.; Kalyabina, Valeriya P.; Kratasyuk, Valentina A.

doi:10.1134/s1995425521030069

Cited by 24 publications

(18 citation statements)

References 108 publications

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“…The bioluminescence bacteria-based assay is commonly used; it has been applied for more than fifty years to monitor the "general" toxicity of complex media [40][41][42][43][44]. The use of the bioluminescence enzymatic assay is a relatively new direction in the toxicology practice [45,46]. As a rule, the enzymatic bioluminescent assay is based on two coupled enzymatic reactions of luminous bacteria (presented in Section 3.2).…”

Section: Introductionmentioning

confidence: 99%

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Sushko

Vnukova

Churilov

et al. 2022

IJMS

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The Gd-containing metallofullerene derivatives are perspective magnetic resonance imaging contrast agents. We studied the bioeffects of a water-soluble fullerene derivative, gadolinium-endohedral fullerenol, with 40–42 oxygen groups (Gd@Fln). Bioluminescent cellular and enzymatic assays were applied to monitor toxicity and antioxidant activity of Gd@Fln in model solutions; bioluminescence was applied as a signaling physiological parameter. The Gd@Fln inhibited bioluminescence at high concentrations (>2·10−1 gL−1), revealing lower toxicity as compared to the previously studied fullerenols. Efficient activation of bioluminescence (up to almost 100%) and consumption of reactive oxygen species (ROS) in bacterial suspension were observed under low-concentration exposure to Gd@Fln (10−3–2·10−1 gL−1). Antioxidant capability of Gd@Fln was studied under conditions of model oxidative stress (i.e., solutions of model organic and inorganic oxidizers); antioxidant coefficients of Gd@Fln were determined at different concentrations and times of exposure. Contents of ROS were evaluated and correlations with toxicity/antioxidant coefficients were determined. The bioeffects of Gd@Fln were explained by hydrophobic interactions, electron affinity, and disturbing of ROS balance in the bioluminescence systems. The results contribute to understanding the molecular mechanism of “hormetic” cellular responses. Advantages of the bioluminescence assays to compare bioeffects of fullerenols based on their structural characteristics were demonstrated.

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Section: Introductionmentioning

confidence: 99%

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Sushko

Vnukova

Churilov

et al. 2022

IJMS

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“…A classic example is the bioluminescent cellular method based on luminous marine bacteria that has been widely applied since the 1960s [ 27 , 28 , 29 , 30 , 31 ]. The enzymatic bioassay variant was first proposed in 1990 for ecotoxicological monitoring [ 31 ]; extensive utilization since the 1980s has highlighted its numerous advantages [ 32 , 33 , 34 , 35 ]. The primary physicochemical bioluminescent mechanisms and the nature of their interactions in bioluminescent systems have been extensively discussed [ 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

et al. 2022

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Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.

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“…Advantages of the enzymatic assay include a possibility to change sensitivity to toxicants by varying the component concentration and composition as well as by constructing coupled polyenzymatic systems [14][15][16][17]. Technological applications of the bioluminescent enzymatic system were reviewed in [18,19].…”

Section: Introductionmentioning

confidence: 99%

Marine Bacteria under Low-Intensity Radioactive Exposure: Model Experiments

Kolesnik

Rozhko

Kudryasheva

2022

IJMS

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Radioactive contaminants create problems all over world, involving marine ecosystems, with their ecological importance increasing in the future. The review focuses on bioeffects of a series of alpha and beta emitting radioisotopes (americium-241, uranium-(235 + 238), thorium-232, and tritium) and gamma radiation. Low-intensity exposures are under special consideration. Great attention has been paid to luminous marine bacteria as representatives of marine microorganisms and a conventional bioassay system. This bioassay uses bacterial bioluminescence intensity as the main testing physiological parameter; currently, it is widely applied due to its simplicity and sensitivity. Dependences of the bacterial luminescence response on the exposure time and irradiation intensity were reviewed, and applicability of hormetic or threshold models was discussed. A number of aspects of molecular intracellular processes under exposure to low-intensity radiation were analyzed: (a) changes in the rates of enzymatic processes in bacteria with the bioluminescent system of coupled enzymatic reactions of NADH:FMN-oxidoreductase and bacterial luciferase taken as an example; (b) consumption of an intracellular reducer, NADH; (c) active role of reactive oxygen species; (d) repairing of the DNA damage. The results presented confirm the function of humic substances as natural radioprotectors.

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Enzymatic Biotesting: Scientific Basis and Application

Cited by 24 publications

References 108 publications

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

Marine Bacteria under Low-Intensity Radioactive Exposure: Model Experiments

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