In vivo cytogenetic effects of a commercially formulated mixture of cypermethrin and quinalphos in mice

Chauhan, Lalit Kumar Singh; Chandra, Satya V.; Saxena, Parul; Gupta, S.K.

doi:10.1016/j.mrgentox.2005.08.004

Cited by 31 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decreases in MI [Agarwal et al, 1994;Chauhan et al, 1999Chauhan et al, , 2005Saxena et al, 2005], PI [Puig et al, 1989], and NDI [Surrallés et al, 1995] were also reported in previous investigations on the cytotoxic potential of insecticides. Superoxide anions can also be responsible for the cytotoxicity of acetamiprid.…”

Section: Discussionsupporting

confidence: 81%

In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes

Kocaman

Topaktaş

2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

Acetamiprid, a neonicotinoid insecticide, is commonly used both in agriculture and domestic areas against a wide range of insects. The potential genotoxicity of a commercial formulation of acetamiprid (Mosetam 20 SP, containing 20% acetamiprid as the active ingredient) on human peripheral blood lymphocytes was examined in vitro by sister chromatid exchange (SCE), chromosomal aberrations (CAs), and micronucleus tests. Cells were treated with 25, 30, 35, and 40 mug/ml of acetamiprid for 24 and 48 hr. Acetamiprid induced SCEs and CAs significantly at all concentrations and treatment times and micronucleus formation was significantly induced at 30, 35, and 40 mug/ml of acetamiprid as compared with both the control and solvent control. Acetamiprid decreased the proliferation index (PI) at the two highest concentrations (35 and 40 mug/ml) for the 24-hr treatment period and only at the highest concentration (40 mug/ml) for the 48-hr treatment period when compared with the control and solvent control. Peripheral lymphocytes exposed to all concentrations of acetamiprid showed significant decreases in mitotic index (MI) and nuclear division index (NDI) for both treatment periods when compared with both the control and solvent control. Furthermore, acetamiprid decreased the MI in both treatment periods, and the NDI only in the 24-hr treatment period to the same extent as the positive control, mitomycin C (MMC). This study presents the first in vitro evidence for the genotoxicity of a commercial formulation of acetamiprid in human peripheral lymphocytes.

show abstract

Section: Discussionsupporting

confidence: 81%

In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes

Kocaman

Topaktaş

2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Many researchers have found various pesticides, including Pro, to cause genotoxicity in peripheral blood lymphocytes of people applying pesticides in agriculture (2,(41)(42)(43)(44). Furthermore, there are many investigations about the genotoxic effects of combinations of different pesticides (45)(46)(47)(48)(49)(50)(51)(52).…”

Section: Discussionmentioning

confidence: 99%

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

Rasgele

2014

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Pesticides are one of the most potent environmental contaminants, which accumulate in biotic and abiotic components of ecosystems. Acetamiprid (Acm), a neonicotinoid insecticide, and Propineb (Pro), a dithiocarbamate fungicide, are widely used to control sucking insects and fungal infections on crops, respectively. The present study was undertaken to investigate the genotoxic effects of these compounds, individually and in mixtures, in mouse germ cells by using the sperm morphology assay. Mice were injected intraperitoneally with 0.625, 1.25, and 2.50 µg mL -1 of Acm, 12.5, 25, and 50 µg mL -1 of Pro, and their mixture at the same concentrations over 24 and 48 h. Acm did not significantly increase the percentage of abnormal sperm at any concentration. The frequency of abnormal sperm significantly increased after 24 and 48 h of exposure to 50 µg mL -1 of Pro. The mixtures of 2.50 µg mL -1 of Acm and 50 µg mL -1 of Pro induced sperm abnormalities antagonistically both after 24 and 48 h of exposure. Results suggest that Acm was non-genotoxic for mouse germ cells, while Pro may have been a germ cell mutagen due to the observed increase in the frequency of sperm abnormalities. However, to gain better insight into the mutagenicity and DNA damaging potential of both of these pesticides, further studies at molecular level should be done.

show abstract

“…An increment of the number of MN is an indirect marker of structural and numeric chromosomal abnormalities caused in the cells by many agents. As chromosome aberrations and MN formation are adjunct cytogenetic indicators of genotoxicity, it was concluded that quinalphos might be capable of inducing significant chromosome damage (Chauhan et al, 2015 ). Rupa et al ( 1991 ) also demonstrated that quinalphos can induce chromosome breaks/fragments in mice and human lymphocytes.…”

Section: Resultsmentioning

confidence: 99%

Chronic exposure to quinalphos shows biochemical changes and genotoxicty in erythrocytes of silver barb, Barbonymus gonionotus

Sadiqul

Kabir

Ferdous

et al. 2017

Interdisciplinary Toxicology

View full text Add to dashboard Cite

An in vivo study was carried out on the freshwater fish Barbonymus gonionotus to evaluate the genotoxic effects of the organophosphate quinalphos. The fish were exposed to sub-lethal doses of quinalphos (0%, 10%, 25%, and 50% of LC50) for a period of 30 days. Analysis of biochemical characteristics (protein and lipid contents of different organs), nuclear abnormalities of erythrocytes (NAE) and morphological abnormalities of erythrocytes (MAE) were performed on peripheral erythrocytes sampled at post-treatment intervals of 0 and 30 days. The biochemical results revealed a significant dose-dependent decline in protein and lipid contents and increase in the frequencies of NAE as well as MAE. Our findings also confirmed that the morphological deformations of erythrocytes in addition to NAE on fish erythrocytes in vivo are effective tools in determining the potential genotoxicity of organophosphates.

show abstract

In vivo cytogenetic effects of a commercially formulated mixture of cypermethrin and quinalphos in mice

Cited by 31 publications

References 18 publications

In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes

In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

Chronic exposure to quinalphos shows biochemical changes and genotoxicty in erythrocytes of silver barb, Barbonymus gonionotus

Contact Info

Product

Resources

About