Chronic exposure to quinalphos shows biochemical changes and genotoxicty in erythrocytes of silver barb, Barbonymus gonionotus

Abstract

An in vivo study was carried out on the freshwater fish Barbonymus gonionotus to evaluate the genotoxic effects of the organophosphate quinalphos. The fish were exposed to sub-lethal doses of quinalphos (0%, 10%, 25%, and 50% of LC50) for a period of 30 days. Analysis of biochemical characteristics (protein and lipid contents of different organs), nuclear abnormalities of erythrocytes (NAE) and morphological abnormalities of erythrocytes (MAE) were performed on peripheral erythrocytes sampled at post-treatment intervals of 0 and 30 days. The biochemical results revealed a significant dose-dependent decline in protein and lipid contents and increase in the frequencies of NAE as well as MAE. Our findings also confirmed that the morphological deformations of erythrocytes in addition to NAE on fish erythrocytes in vivo are effective tools in determining the potential genotoxicity of organophosphates.

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

“…Moreover, Al-Obaidi and Al-Shawi [72] reported findings similar to our results; they reported that FLX has genotoxic potential and can induce MN in polychromatic erythrocytes in the bone marrow of male rats. However, the origins of these morphological alterations of the nucleus have been found to arise from genotoxic events caused by exposure to xenobiotic agents [73] .…”

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

“…Dacrocyte (Da, Tr): [4,47,77,87,96,129,140,152,153]. Schistocyte (Sch, Sh): [5,69,77,130,154,155]. Codocyte (C): [44,93].…”

Interaction of 2-Hydroxyquinoxaline (2-HQ) on Soil Enzymes and Its Degradation: A Review

“…Despite extensive usage of quinalphos in agriculture for control of pests over crops, inhibition of acetylcholinesterase (AChE) was reported in non-target organisms by quinalphos (Chebbi and David, 2009;Kopecka et al, 2004;Pan and Dutta, 1998;Silman and Sussman, 2005;Vig et al, 2006). Quinalphos adversely influence on blood and brain esterase activity in chickens (Vairamuthu and Thanikachalam, 2003), fish fertility efficiency in adult male rats (Sarkar et al, 2000) and also genotoxicity to silver barb (Sadiqul et al, 2017). A 3-year (2000)(2001)(2002) evaluation report (Teixeira et al, 2004) of forensic pesticide and herbicide intoxications in Portugal indicated about 29% of total cases due to quinalphos.…”