In vivo bone metabolism and ex vivo bone marrow osteoprogenitors in vitamin D-deprived pigs

Denis, Isabelle; Cournot, Giulia; Lacroix, H.; Colin, Colette; Zérath, E.; Pointillart, Alain

doi:10.1016/s8756-3282(00)00257-x

Cited by 12 publications

(11 citation statements)

References 33 publications

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“…Furthermore, these data provide an important clue as to how vitamin D hormone acts in bone, not by suppressing the osteoclastogenesis-supporting microenvironment ("soil"), represented by RANKL, but by decreasing the number of osteoclast progenitors, that is, "seeds" of osteoclasts, in BM. In this respect, it is interesting to note that vitamin D deprivation in growing pigs is associated with an enlarged pool of osteoclasts, (20) a mirror image of the situation in our vitamin D-treated mice. It also is noted that estrogen deficiency is not associated with substantial up-regulation of RANKL in bone, at least at the RNA level ( Fig.…”

mentioning

confidence: 67%

Vitamin D Hormone Inhibits Osteoclastogenesis In Vivo by Decreasing the Pool of Osteoclast Precursors in Bone Marrow

Shibata

Shiraishi²,

Sato

et al. 2002

Journal of Bone and Mineral Research

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confidence: 67%

Vitamin D Hormone Inhibits Osteoclastogenesis In Vivo by Decreasing the Pool of Osteoclast Precursors in Bone Marrow

Shibata

Shiraishi²,

Sato

et al. 2002

Journal of Bone and Mineral Research

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“…These cells developed from porcine nonadherent marrow cells, as shown by other investigators. (32)(33)(34) Daidzein, like estradiol reduced the number of porcine osteoclasts that differentiated in response to 1,25(OH) 2 D 3 , and this effect was reversed by the estrogen antagonist ICI 182,780. (35) The effect of 17␤-estradiol and ICI 182,780 on osteoclast formation is in agreement with other observations reported in human, rat, and mouse bone marrow cultures.…”

Section: Discussionmentioning

confidence: 99%

Down-Regulation of Osteoclast Differentiation by Daidzein via Caspase 3

Rassi

Lieberherr

Chaumaz³

et al. 2002

Journal of Bone and Mineral Research

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Phytoestrogens are plant-derived compounds with estrogen-like activity. Phytoestrogen-rich diets may prevent postmenopausal osteoporosis and these molecules maintain bone mass in ovariectomized animals. We compared the effects of the isoflavone daidzein, which has no action on tyrosine kinases, and 17␤-estradiol on the development and activity of osteoclasts in vitro. Nonadherent porcine bone marrow cells were cultured on dentine slices or on culture slides in the presence of 10

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“…Pigs appear to be more suitable than rodents for investigating the effects of nutritional factors on the skeleton, since bone remodeling occurs in pigs (Iwaniec and Crenshaw 1998) but not in rodents. Moreover, recent data have shown that porcine bone marrow contains large numbers of osteoclast progenitors (Galvin et al 1996;Scheven et al 1998;Denis et al 2000;Rassi et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Modulation of osteoclastogenesis in porcine bone marrow cultures by quercetin and rutin

Rassi¹,

Lieberherr²,

Chaumaz³

et al. 2005

Cell Tissue Res

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Flavonols, in contrast to soybean isoflavones, are the most abundant phytoestrogens in western diets, being present in onions, beans, fruits, red wine, and tea. They may protect against atherosclerosis, inhibit certain cancer cell types, and reduce bone resorption. The most widely distributed flavonol is quercetin, which occurs mainly as its glycoside, rutin, but data are very scarce regarding the precise mechanism of action of these compounds on bone-resorbing cells at concentrations similar to those detected in human plasma. We have therefore investigated the effects of nanomolar concentrations of quercetin and rutin on the development and activity of osteoclasts in vitro compared with the effects of 17beta-estradiol. Nonadherent porcine bone marrow cells were cultured on dentine slices in the presence of 10 nM 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), with or without 10 nM quercetin, 10 nM rutin or 10 nM 17beta-estradiol for 11 days. Multinuclear TRAP+ cells that resorbed dentine (osteoclasts) developed in the presence of 1,25(OH)2D3, but their number was significantly reduced by quercetin, rutin, and 17beta-estradiol (P < 0.05). Like 17beta-estradiol, both flavonols also significantly reduced resorption (P<0.05) as assessed by the size of pits resorbed on dentine slices. Osteoclasts and osteoclast progenitors contained estrogen receptor alpha (ERalpha), ERbeta, and RANK proteins. Both flavonols increased nuclear ERbeta protein and decreased ERalpha protein of osteoclast progenitors. Moreover, rutin reduced RANK protein, whereas 17beta-oestradiol and quercetin promoted apoptosis by cleavage of caspase-8 and caspase-3. All the effects of flavonols were reversed by 1 microM ICI 182,780, an estrogen antagonist. Thus, the anti-resorbing properties of flavonols are mainly mediated by ER proteins through the inhibition of RANK protein or the activation of caspases.

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In vivo bone metabolism and ex vivo bone marrow osteoprogenitors in vitamin D-deprived pigs

Cited by 12 publications

References 33 publications

Vitamin D Hormone Inhibits Osteoclastogenesis In Vivo by Decreasing the Pool of Osteoclast Precursors in Bone Marrow

Vitamin D Hormone Inhibits Osteoclastogenesis In Vivo by Decreasing the Pool of Osteoclast Precursors in Bone Marrow

Down-Regulation of Osteoclast Differentiation by Daidzein via Caspase 3

Modulation of osteoclastogenesis in porcine bone marrow cultures by quercetin and rutin

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