In Vivo and In Vitro Evaluation of Preventive Activity of Inflammation and Free Radical Scavenging Potential of Plant Extracts from Oldenlandia corymbosa L.

Abstract

Aims: The present study evaluates the anti-inflammatory and antioxidant activity of the crude dichloromethane (CDCME), ethyl acetate (CEAE), and methanol (CMeE) extracts from the plant Oldenlandia corymbosa L. Background:Oldenlandia species have been popular among the people of the Indian subcontinent to treat several types of internal and external inflammation for a long time. Plant decoctions have been used to battle inflammation in cases of tonsilitis, pneumonia and cholecystitis, among others. Objective: The present work designed to demonstrate the properties of the previously mentioned plant extracts to prevent inflammation both in vivo and in vitro. This work is the first investigation of such extracts from this species and their relationship with anti-inflammatory activity. Method: The anti-inflammatory properties of the Oldenlandia corymbosa L. extracts were evaluated in vitro with the Red Blood Cell (RBC) membrane stabilization method and the protein denaturation method and in vivo with the carrageenan-induced paw oedema method. Furthermore, the free radical scavenging activity of the extracts was carried out with the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical oxidation, total antioxidant capacity and iron reduction assay. Result: Both in vivo and in vitro studies showed that CDCME had the most predominant effects with the value of 80.5% for RBC membrane stabilization, 60% for inhibition of protein denaturation at the concentration of 1000 µg/mL and 63.28% (after 3 h, * p < 0.05) for inhibition of paw oedema (300 mg/kg bwt) compared to carrageenan-induced mice. The free radical scavenging activity was studied by DPPH, total antioxidant and reducing activity assay. CDCME showed scavenging activity in all the methods and an IC50 value of 473.86 µg/mL for DPPH method. Conclusions: The findings of the study remarked that CDCME of the plant has strong anti-inflammatory and antioxidant effects that validate the traditional use of the plant to get remedy from pain. Other: The plants Oldenlandiacorymbosa Linn were provided by the Bangladesh Council of Scientific and Industrial Research Laboratory campus, Rajshahi, Bangladesh. Experiments on animals were conducted by ethical permission of Institute of Biological Sciences, University of Rajshahi, Bangladesh (license no: 225/320-IAMEBBC/IBSc).

Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from Litsea glutinosa: In Vitro and In Vivo Studies

“…The total phenol and flavonoid [ 47 ] content of LGBME were determined spectro-photometrically at 760 nm using Folin–Ciocalteu and aluminum chloride reagent. Total phenolic contents were expressed in terms of gallic-acid equivalent, GAE mg of GA/gm of extract, and the standard curve equation was y = 0.0863x + 0.2372, R 2 = 0.9896.…”

“…In DPPH (2,2-diphenyl-1-picryl-hydrazyl)-free-radical-scavenging-activity assay, LGBME was tested at 517 nm [ 47 ] and the standard curve equation: y = 1.061x + 44.682, R 2 = 0.8082.…”

“…The antioxidant activity of the LGBME was evaluated by the phosphor-molybdenum method [ 47 ]. Absorbance was taken at 695 nm.…”

“…The reducing power of LGBME was measured by the ferric reducing method [ 47 ]. The amount of Fe 2+ complex was then monitored by measuring the formation of Perl’s Prussian blue at 700 nm.…”

Exploring the Antioxidant and Antiproliferative Properties of Flacourtia indica Extracts on Lung Cancer Cells: A Comprehensive Analysis Utilizing GC-MS, Molecular Docking, and PASS Analysis.

“…Where Ac = absorbance of control, As = absorbance of sample Iron Reducing Activity Test According to Bashera et al [17], the activity of the plant extract to prevent oxidation can be determined by the extent of reduction of iron. In this method, the sample are let to undergo reaction with 1% potassium ferrycyanide maintaining pH 6.6 by phosphate buffer.…”