Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from Litsea glutinosa: In Vitro and In Vivo Studies

Abstract

Litsea glutinosa (L. glutinosa) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, L. glutinosa, were examined using non-toxic doses and a phytochemical analysis was executed using gas-chromatography–mass-spectrometry. The in vitro antioxidant study of the L. glutinosa methanolic extract (LGBME) revealed a concentration-dependent antioxidant property. The bark extract showed promising antioxidant effects in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest antioxidant activity was demonstrated at the maximum concentration (50 µg/mL). The IC50 values of the LGBME and BHT were 5.51 and 5.01 µg/mL, respectively. At the same concentration, the total antioxidant capacity of the LGBME was 0.161 µg/mL and the ferric reducing antioxidant power assay result of the LGBME was 1.783 µg/mL. In the cytotoxicity study, the LD50 of the LGBME and gallic acid were 24.93 µg/mL and 7.23 µg/mL, respectively. In the in vivo anticancer-activity studies, the LGBME, particularly at a dose of 150 mg/kg/bw, showed significant cell-growth inhibition, decreased tumor weight, increased mean survival rate, and upregulated the reduced hematological parameters in EAC (Ehrlich’s ascites carcinoma)-induced Swiss albino mice. The highest cell-growth inhibition, 85.76%, was observed with the dose of 150 mg/kg/bw. Furthermore, the upregulation of pro-apoptotic genes (p53, Bax) and the downregulation of anti-apoptotic Bcl-2 were observed. In conclusion, LGBME extract has several bioactive phytoconstituents, which confirms the antioxidant and anticancer properties of L. glutinosa.

Profil Kandungan Kimia, Fenolik Total, dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Litsea firma (Blume) Hook F

“…Penelitian aktivitas antioksidan pada tumbuhan genus Litsea sebelumnya dilaporkan dari ekstrak daun dan kulit batang L. glutinosa (Shafiq et al, 2022), kulit batang L. elliptica (Mariani et al, 2021), kayu batang L. monopelata (Arfan et al, 2008), dan ekstrak L. cubeba (Hwang et al, 2005). Akan tetapi, penentuan aktivitas antioksidan dari semua bagian tumbuhan Litsea firma belum pernah dilaporkan.…”

The Potential Role of MUC16 (CA125) Biomarker in Lung Cancer: A Magic Biomarker but with Adversity

“…For analyzing mutation distribution in the MUC16 gene, 22 tissue samples (10 pairs of NSCLC and their neighboring nonmalignant tissues, in addition to two carcinogenic tissues) and 10 cell lines of lung cancer were chosen for captured target gene sequencing. Total single-nucleotide polymorphism (SNP) and data of insertion and deletion (InDel) from each individual were collected and compared between the two groups [ 28 , 34 , 42 ]. After all shared SNPs and InDels were removed, the MUC16 up-regulated group and the MUC16 unchanged/down-regulated group displayed distinct patterns.…”

GC-MS Characterization, in Vitro Antioxidant and Anti-Inflammatory Activities of Essential oil from the Leaves of Litsea balansae Lecomte

“…4 In traditional medicine, the leaves, fruits, or essential oils derived from Litsea plants have been employed to treat various ailments, including digestive issues, respiratory conditions, and skin disorders. 4 These plants contain terpenes and phenolic compounds with broad-spectrum activity such as anti-inflammatory, 2,4 antioxidant, 5,6 antimicrobial, 7 and anticancer 8,9 properties.…”