In vivo and in situ measurements of spectroscopic and photosynthesis properties of undetached maize leaves using the open photoacoustic cell technique

Pereira, Antônio Carlos; Prioli, L. M.; Silva, William J. da; Neto, Graciliano de Oliveira; Vargas, H.; Cella, N.; Alvarado‐Gil, J. J.

doi:10.1016/0168-9452(94)90237-2

Cited by 16 publications

(10 citation statements)

References 21 publications

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“…In previous studies, the entire plant was kept in dark conditions during the measurements, with the exception of a 3 mm diameter area of illumination on the leaf area being studied [13,14]. It is important to note that in our work, the entire plant received full illumination while the measurements were being taken.…”

Section: Methodsmentioning

confidence: 98%

“…A small sample of a detached leaf is placed inside the chamber and is studied by monitoring its response when illuminated by a modulated light beam [9,10]. The open photoacoustic cell (OPC) technique uses the same kind of microphone; however, in OPC, the microphone is sealed at the surface of the intact, still attached leaf [13,14]. The modulated light beam hits the leaf in the same place where the microphone is attached.…”

mentioning

confidence: 99%

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Benefits of the Mycorrhizal Fungi in Tomato Leaves Measured by Open Photoacoustic Cell Technique: Interpretation of the Diffusion Parameters

et al. 2008

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The open photoacoustic cell technique was used to measure tomato leaves from plants with and without mycorrhizal fungi (Glomus fasciculatum). Based on measurement of the photobaric contribution of the photoacoustic signal, the exponential parameter of the diffusion behavior for this contribution was calculated. From this value, the oxygen diffusion coefficient was derived. The changes in the oxygendiffusion-coefficient-dependent exponential parameter are statistically significant ( p < 0.05) and are consistent with the expected benefits of mycorrhizal symbiosis. Potentially similar results obtained from the photothermal contribution are discussed.

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Section: Methodsmentioning

confidence: 98%

mentioning

confidence: 99%

Benefits of the Mycorrhizal Fungi in Tomato Leaves Measured by Open Photoacoustic Cell Technique: Interpretation of the Diffusion Parameters

et al. 2008

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“…The response of plants to toxic substances was studied by Marquezini et al [18] who monitored the effects of the toxic action of aluminum on maize specimens through spectroscopic measurements performed with the OPC set-up. Pereira et al [19] also worked on the characterization of maize leaves, studying differences in the spectra of mutants and wild maize types.…”

Section: Open Photoacoustic Cell (Opc)mentioning

confidence: 98%

Open Photoacoustic Cell: Applications in Plant Photosynthesis Studies

Mesquita

Mansanares

Silva

et al. 2006

Instrumentation Science & Technology

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In this article, we review the applications of the open photoacoustic cell (OPC) technique in studies of photosynthetic activity in plant leaves. The ability to perform in vivo and in situ measurements makes this technique particularly suitable for monitoring photosynthesis of plants subjected to specific treatments. Therefore, our objective in the present article is to describe, in more detail, the principles of the OPC technique and to follow the historical path of its applications related to the study of plants and photosynthesis, including recent results.It will be shown that the technique has been used to observe spectroscopic responses to herbicide application and to soil toxicity, in the study of photosynthesis induction, gas exchange, photochemical loss, photoinhibition, photosynthesis saturation, and photosynthetic responses to changes in parameters such as temperature, atmospheric conditions, and nutrient availability. Recent results have demonstrated its usefulness in the determination of the action spectrum directly from oxygen evolution measurements.

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“…They also observed dehydration effects in the photosynthetic activity of a detached leaf, verifying that the possibility of performing measurements with the leaf attached to the plant was an essential feature of the OPC method. Using the OPC, in 1994 Pereira et al [20] showed the correlation between the photosynthetic activity and the chlorophyll content of the maize leaves. Oxygen evolution rates indicated that green leaves have a photosynthetic activity up to three times superior to that of chlorophylldeficient samples.…”

Section: Opc and In Vivo Plant Photosynthesis Researchmentioning

confidence: 99%

“…Still working with maize leaves, Pereira et al [20] investigated differences in the spectra of mutants and wild maize types.…”

Section: Open Photoacoustic Cell (Opc)mentioning

confidence: 99%

How Can Physicists Study Photosynthesis? History and Applications of the Photoacoustic Technique

Barja¹

2000

Physicae

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This short review presents the photoacoustic technique applied to the study of photosynthesis. It is known that plants kept in the dark for several hours stop photosynthetic activity. After this dark-adaptation, light incidence gives rise to the socalled photosynthetic induction (restart of the photosynthetic processes). The Open Photoacoustic Cell described here has been used at the Laboratory of Photothermal Spectroscopy (IFGW, UNICAMP) to investigate the kinetics of the photosynthetic induction. Recent work developed at this laboratory includes in vivo and in situ measurements in eucalyptus leaves from different species and subjected to different conditions. With the photoacoustic technique, we can analyze the parameters that influence photosynthesis, such as temperature and intensity of the incident radiation.

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In vivo and in situ measurements of spectroscopic and photosynthesis properties of undetached maize leaves using the open photoacoustic cell technique

Cited by 16 publications

References 21 publications

Benefits of the Mycorrhizal Fungi in Tomato Leaves Measured by Open Photoacoustic Cell Technique: Interpretation of the Diffusion Parameters

Benefits of the Mycorrhizal Fungi in Tomato Leaves Measured by Open Photoacoustic Cell Technique: Interpretation of the Diffusion Parameters

Open Photoacoustic Cell: Applications in Plant Photosynthesis Studies

How Can Physicists Study Photosynthesis? History and Applications of the Photoacoustic Technique

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