Lhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 degrees C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 degrees C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the non-transgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature.
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5-Experimental SurgeryActa Cir Bras. 2017;32(2):125-139 Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C -surgical procedures without tendon lesion and with simulation of hAM application; I -surgical procedures, tendon injury and simulation of hAM application; T -surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.
Introdução: A mobilização neural e o alongamento muscular estático têm o objetivo de promover a amplitude de movimento. Objetivo: Comparar a influência do alongamento muscular e da mobilização neural na força isométrica de quadríceps. Metodologia: Participaram 39 voluntários, gênero masculino, entre 18 e 27 anos. Foram divididos aleatoriamente em três grupos com 13 indivíduos cada. Um grupo submetido ao alongamento estático de 30 segundos (GAE), um à mobilização neural (GMN) e um grupo controle (GC) que não fez nenhuma intervenção. O nível de significância estatística adotado foi p≤0,05. Resultados: Houve melhora significativa da força muscular no GMN e diferença importante na força antes e após os atendimentos, comparando-se o grupo movimentação neural com o alongamento e com o controle. Conclusões: A mobilização neural foi efetiva para aumentar a força muscular de quadríceps e após a realização do alongamento estático houve redução da força.
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