In vitrohaemopoiesis induced in a rainbow trout pronephric stromal cell line (TPS)

Diago, M.L.; López‐Fierro, P.; Razquin, B.; Villena, A.

doi:10.1006/fsim.1997.0121

Cited by 15 publications

(12 citation statements)

References 31 publications

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“…Previously, the addition of head kidney leucocytes to RTS34st cultures was found to cause the growth of macrophages [15], and their addition to RTG-2 cultures was reported to support haemopoiesis, although not as well as the trout pronephros stromal cell line (TPS) [34]. The results of this study with RTS11 show that both RTS34st and RTG-2 have the capacity to enhance macrophage growth.…”

Section: Rts11 In Co-culture or Conditioned Mediummentioning

confidence: 77%

Effect of corticosteroids on viability and proliferation of the rainbow trout monocyte/macrophage cell line, RTS11

Pagniello

Bols

Lee

2002

Fish & Shellfish Immunology

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Section: Rts11 In Co-culture or Conditioned Mediummentioning

confidence: 77%

Effect of corticosteroids on viability and proliferation of the rainbow trout monocyte/macrophage cell line, RTS11

Pagniello

Bols

Lee

2002

Fish & Shellfish Immunology

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“…Haemopoietic activity has been previously described when the TPS cell line is co-cultured with pronephric cell suspensions (Diago et al, 1998). Such co-cultures are able to support long-term haemopoiesis when maintained in the same flask (without passages), and the medium renewed weekly.…”

Section: Resultsmentioning

confidence: 97%

“…Long-term haemopoietic cultures obtained from pronephric explants (Diago et al, 1993), and the co-cultures established with TPS cells and pronephric cell suspensions (Diago et al, 1998), contained epithelioid cells, which were also considered to be of possible endothelial origin. Our present results confirm this suggestion, but the epithelioid cells need some stimuli to promote their di#erentiation into endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…The methodology for the establishment of co-cultures of TPS cells and pronephric cell suspensions have been previously described (Diago et al, 1998). Briefly, passages from 40-50 of the TPS cell line were co-cultured with trout pronephric cell suspensions.…”

Section: Tps-pronephros Co-culturesmentioning

confidence: 99%

“…We have developed a cell line, the TPS (trout pronephric stroma) from the pronephros of rainbow trout (Diago et al, 1995), which is able to support long-term haemopoiesis when co-cultured with pronephric cell suspensions (Diago et al, 1998). In this paper we describe spontaneous angiogenesis processes in vitro that occurred in TPS cultures and in haemopoietic cultures obtained from TPS-pronephric cell suspension co-cultures.…”

Section: Introductionmentioning

confidence: 97%

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Spontaneous in vitro angiogenesis in a trout pronephric stromal cell line (TPS), and in TPS-haemopoietic co-cultures

Diago

López‐Fierro

Razquin

et al. 2000

Fish & Shellfish Immunology

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Differential compartmentalization of memory B cells versus plasma cells in salmonid fish

Kaattari

2013

Eur J Immunol

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The disposition of teleost memory and plasma cells (PCs) has essentially been unexplored. As the organization of the teleost immune system differs significantly from that of mammals (i.e. no bone marrow or lymph nodes, hematopoietic anterior kidney), this disposition could be essential in understanding how comparable functions are achieved. To address this question, the primary and secondary antibody-secreting cell, B memory cell, and antibody responses to T-independent and T-dependent antigens were analyzed in trout. Although the TI and TD antibody responses did not differ substantively from one another, the secondary responses to both were significantly prolonged compared with the primary responses. Logarithmic increases in titer and affinity were achieved for both antigens during the primary, with only modest increases during the secondary response. Antibody-secreting cells, both PCs and plasmablasts, quantitatively paralleled antibody production, with antibody-secreting cells skewing to the hematopoietic anterior kidney for both antigens. However, the enhanced antigen-inducible response of immune fish (indicative of the memory pool) skewed to the peripheral blood and spleen. This pattern of memory versus PC disposition parallels that observed in mammals even though the organization of the respective immune systems differs considerably. Keywords: Anterior kidney r Memory cells r Plasma cells r Trout IntroductionTeleost memory has been defined by the development of a differentially heightened responsive state to antigen, albeit lacking in a number of mammalian features. Primary immunization can, thus, lead to increased immunogen sensitivity [1], enhanced in vivo [2][3][4][5][6] and in vitro [6][7][8] antibody responsiveness, expansion of antigen-sensitive precursor pools [9] and, most importantly, anamestic responses to pathogens [4,5,10,11] and immunoprophylaxis [4,[12][13][14]. Anamestic features absent from teleost responses include isotype switching [15] (although they possess multiple isotypes [16]), relatively modest somatic mutaCorrespondence: Dr. Stephen L. Kaattari e-mail: kaattari@vims.edu tion [17][18][19] and affinity maturation [20][21][22] as compared with the IgG responses of mammals. Intriguingly, characteristics of teleost memory B cells (MBCs) [9] parallel those associated with a class of mammalian MBCs. Recent evidence reveals that human MBCs can include unswitched, mutated IgM + cells [23]; nonmutated, CD27 + IgM + cells [24], those induced by T-independent (TI) antigens [25,26], preimmune diversified repertoires [27] (as has recently been posed for the catfish [19]), or arise independently of GCs [27,28]. Together, these previous studies prompt a renewed focus on the critical elements of μ specific memory, both in teleosts and mammals. For example, perhaps the requisites for exceptionally high-titered, extensively mutated, high-affinity responses in * These authors contributed equally to this work. [32][33][34], which home to their respective hematopoietic tissues, even though the organs ...

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In vitrohaemopoiesis induced in a rainbow trout pronephric stromal cell line (TPS)

Cited by 15 publications

References 31 publications

Effect of corticosteroids on viability and proliferation of the rainbow trout monocyte/macrophage cell line, RTS11

Effect of corticosteroids on viability and proliferation of the rainbow trout monocyte/macrophage cell line, RTS11

Spontaneous in vitro angiogenesis in a trout pronephric stromal cell line (TPS), and in TPS-haemopoietic co-cultures

Differential compartmentalization of memory B cells versus plasma cells in salmonid fish

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