Spontaneous in vitro angiogenesis in a trout pronephric stromal cell line (TPS), and in TPS-haemopoietic co-cultures

Diago, M.L.; López‐Fierro, P.; Razquin, B.; Villena, A.

doi:10.1006/fsim.1999.0224

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…This type of cells had more numerous cytoplasmic vesicles (diameter 0.6-2 mm) than the limiting-subcapsular cells. The content of these vesicles could have a role in chemotactic and=or of erythropoiesis stimulation (Diago et al, 2000), in vessel contraction (Morales et al, 1990) and adrenal gland regulation.…”

Section: Head Kidneymentioning

confidence: 99%

Adaptation of fish lymphomyeloid organs to polar water

Romano

Ceccariglia

Abelli

et al. 2004

Chemistry and Ecology

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Lymphomyeloid organs of three common Antarctic fish species, Trematomus bernacchii, Trematomus nicolai and Chionodraco hamatus, were analysed.Contrary to species living in temperate sea water, the thymus of polar fishes were flattened, incompletely lobated and scarcely distinguishable by normal histology into cortical and medullary regions. Functional regionalisation, however, was suggested by differences in the sizes of thymocytes from the outer to the inner thymus zone. Another particularity was observed in the thymus of Trematomus species: next to lymphocytes, numerous erythroid cells circulated and differentiated in the parenchyma. Only two main types of epithelial cells could be found by cytological analysis: (i) limiting cells that surround the haematopoietic tissue and (ii) reticular cells that constitute the frame where the lymphoid and erythroid cells can proliferate and differentiate. The reticular cells could not be distinguished in cortical and medullary subtypes as observed in temperate-water fish. Numerous Hassall's corpuscles, probably with a scavenging role, were also observed in the thymus.The head kidney housed haematopoietic tissue, lacked any excretory tubules, and had a huge blood supply, characteristic of polar fish species. It appeared mainly lymphopoietic in C. hamatus but contemporary erythropoietic and lymphopoietic in Trematomus species. The ultrastructural analysis revealed the presence of both reticular and limiting epithelial cells. Reticular epithelial cells (REC) characteristically showed numerous vesicles with a granular content and cell debris. Numerous lymphoblasts, lymphocytes and plasma cells were observed among the REC. Erythropoiesis occurred in all polar species analysed, but in C. hamatus the erythroblasts did not differentiate because they had a fast senescence.The spleen appeared mainly erythropoietic, with scarcely developed areas of white pulp, in Trematomus species; the erythropoiesis was scarcely evident in C. hamatus. Small vascular ellipsoids showed numerous melano-macrophages in Trematomus, while large haematopoietic areas were organised around the capillaries in C. hamatus. Utrastructural analysis revealed, in all species examined, two main types of epithelial cells: reticular, close to the ellipsoids, and limiting-subcapsular, which surround the organ. A large blood supply and extended capillary frame were also observed in polar species. The possible adaptation of lymphoid organs to the low temperatures of polar water is discussed.

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Section: Head Kidneymentioning

confidence: 99%

Adaptation of fish lymphomyeloid organs to polar water

Romano

Ceccariglia

Abelli

et al. 2004

Chemistry and Ecology

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“…When they became available, the in vitro immunological experiments were performed in morphologically and functionally distinct catfish cell lines resembling B-cells, T-cells, and monocytes (Vallejo et al, 1991;Miller et al, 1994;Warr, 1997;Stuge et al, Defence responses (mitogenic and antigen cell proliferation, antibodyforming cell and cytotoxic assays) Haemopoiesis and production and effects of haemopoietic growth factors Anderson et al, 1986, 1989Diago et al, 1998Flaño et al, 1998 Identification of dendritic cells Ganassin and Bols, 1996 Angiogenesis in vitro Diago et al, 2000 Long-term cultures from the stroma of pronephros and spleen from rainbow trout…”

Section: Characterization Of the Host Defense Mechanismsmentioning

confidence: 99%

“…Subcultures of haematopoietic and lymphoid organs have been also utilized as a source of stromal cell lines. The only available description of in vitro angiogenesis in fish was done on long-term cultures of a trout pronephric stromal cell line (Diago et al, 2000).…”

Section: Tissue Cultures Of Organ Explants Including Epithelial and C...mentioning

confidence: 99%

Untitled

Villena

2003

Reviews in Fish Biology and Fisheries

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page 111 Scope 112 Diseases constrain the development of aquaculture 112 Aetiology of aquatic animal diseases 113 Application of cell culture methods to protect aquatic animal health 113 Nutritional diseases and the relationship between feeding and immunocompetence 113 Toxicological diseases 114 Infectious diseases 116 Cell culture methods in diagnosis of infectious diseases Safe use of drugs for disease control Study of pathogenicity mechanisms 118 Characterization of the host defense mechanisms 122 Vaccination and immunostimulation 126 Disease resistant strains of aquatic animals 127 Needs for the further development of fish and shellfish cell culture 127 Availability of cell lines and of cell culture methods Standardization of cell and tissue culture methods Validation of in vitro assays Conclusion 130 Acknowledgements 131 References 131

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Development and characterization of an endothelial cell line from the bulbus arteriosus of walleye, Sander vitreus

Chen

Lee

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Spontaneous in vitro angiogenesis in a trout pronephric stromal cell line (TPS), and in TPS-haemopoietic co-cultures

Cited by 4 publications

References 21 publications

Adaptation of fish lymphomyeloid organs to polar water

Adaptation of fish lymphomyeloid organs to polar water

Untitled

Development and characterization of an endothelial cell line from the bulbus arteriosus of walleye, Sander vitreus

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