In vitro toxicity, genotoxicity and antigenotoxicity of Nigella sativa extracts from different geographic locations

Nguyen, Thi‐Thuy‐Dung; Talbi, Hayat; Hilali, A.; Anthonissen, Roel; Maes, A.; Verschaeve, Luc

doi:10.1016/j.sajb.2019.02.015

Cited by 14 publications

(13 citation statements)

References 50 publications

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“…Similarly, in our study, the effects of N. sativa oil on micronucleus formation in healthy human lymphocyte cell culture at concentrations of 1, 5 and 10 µg/mL were examined and the genotoxic effects of CP used as positive control were reduced by N. sativa oil in all three concentrations. Although N. sativa oil concentrations and cell type used in our study were different from those of Nguyen et al 26 , similar results were found. However, the location of the samples, the test and other test conditions may affect the research result.…”

Section: Discussionsupporting

confidence: 80%

“…According to the results of this study, it was observed that combined oral administration of N. sativa oil and fish oil-N. sativa oil combined with anti-inflammatory and antioxidant activity reduced liver and kidney damage. Nguyen et al 26 in Morocco investigated the in vitro cytotoxicity, genotoxicity and antigenotoxicity of aqueous plant extracts from three different regions (Erfoud, Fkih ben Salah, Settat) by the neutral red uptake test in human C3A cells, the bacterial Vitotox, Ames assays, comet assay and micronucleus test. N. sativa seed extracts showed varying degrees of antigenotoxicity depending on where the test specimens came from.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of In Vitro Antigenotoxic Effect of Nigella Sativa Oil

Zor¹,

Kurtoğlu²

2020

tjps

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ÖZAmaç: Siklofosfamid, antineoplastik ve immünosüpresif ajan olarak yaygın olarak kullanılan alkilleyici bir ajandır. Siklofosfamid'in genotoksisitesi çeşitli in vivo ve in vitro sistemde çalışılmış ve rutin olarak genotoksisite testlerinde pozitif kontrol olarak kullanılmaktadır. Geleneksel tıp da Nigella sativa L. (N. sativa), Ranunculaceae familyasından olup günümüzde başta Doğu Akdeniz ülkeleri olmak üzere birçok ülkede yaygın olarak yetişen ve hem baharat hem de halk ilacı olarak Dioscorides zamanından beri kullanılan bir bitkidir. Bu çalışmada Siklofosfamid'in genotoksik etkilerine karşı çörek otu yağının değişik konsantrasyonlardaki koruyucu etkilerininin mikronükleus testi ile gösterilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu amaçla, sağlıklı hücreler 1, 5, 10 µg/mL konsantrasyonlarında N. sativa yağı ile ve pozitif kontrol olarak Siklofosfamid ile 68 saat boyunca in vitro muamele edildi. Daha sonra ise mikronükleuslar sayıldı. Bulgular: N. sativa yağının 1, 5, 10 µg/mL konsantrasyonlarındaki uygulaması negatif kontrolle karşılaştırıldığında mikronükleus frekansında anlamlı bir artış gözlenmedi. N. sativa yağı ve Siklofosfamid'in birlikte uygulandığı gruplarda her üç konsantrasyonda da (1, 5, 10 µg/mL) Siklofosfamid grubuna göre mikronükleus sayısında azalma olduğu görüldü. Sonuç: N. sativa yağının genotoksisite ajanlarına karşı in vitro koruyucu etkilerinin olabileceği gösterilmiştir. N. sativa yağının genotoksisiteyi giderici etkilerinin mekanizmasının anlaşılması için daha fazla çalışmaya ihtiyaç vardır. ABSTRACTObjectives: Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (N. sativa), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of N. sativa oil at different concentrations against the genotoxic effects of CP by micronucleus test. Materials and Methods: For this purpose, healthy cells were treated in vitro with N. sativa oil at concentrations of 1, 5, 10 µg/mL and CP as positive control for 68 hours. The micronuclei were then counted. Results: No significant increase in micronucleus frequency was observed when the application of N. sativa oil at concentrations of 1, 5, 10 µg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 µg/mL) compared to the CP group in the groups treated with N. sativa oil and CP. Conclusion: It has been shown that N. sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of N. sativa oil.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Assessment of In Vitro Antigenotoxic Effect of Nigella Sativa Oil

Zor¹,

Kurtoğlu²

2020

tjps

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“…Here further evidence is essential. Insufficient evidence is available in the context of uses as an adjuvant medication (Nguyen et al, 2019). Overall, safety is High.…”

Section: Nigella Sativa L -Ranunculaceae (Seeds)mentioning

confidence: 99%

COVID-19: Is There Evidence for the Use of Herbal Medicines as Adjuvant Symptomatic Therapy?

et al. 2020

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Background: Current recommendations for the self-management of SARS-Cov-2 disease (COVID-19) include self-isolation, rest, hydration, and the use of NSAID in case of high fever only. It is expected that many patients will add other symptomatic/adjuvant treatments, such as herbal medicines. Aims: To provide a benefits/risks assessment of selected herbal medicines traditionally indicated for "respiratory diseases" within the current frame of the COVID-19 pandemic as an adjuvant treatment. Method: The plant selection was primarily based on species listed by the WHO and EMA, but some other herbal remedies were considered due to their widespread use in respiratory conditions. Preclinical and clinical data on their efficacy and safety were collected from authoritative sources. The target population were adults with early and mild flu symptoms without underlying conditions. These were evaluated according to a modified PrOACT-URL method with paracetamol, ibuprofen, and codeine as reference drugs. The benefits/risks balance of the treatments was classified as positive, promising, negative, and unknown. Results: A total of 39 herbal medicines were identified as very likely to appeal to the COVID-19 patient. According to our method, the benefits/risks assessment of the herbal medicines was found to be positive in 5 cases (Althaea officinalis, Commiphora molmol, Glycyrrhiza glabra, Hedera helix, and Sambucus nigra), promising in 12 cases (Allium sativum, Andrographis paniculata, Echinacea angustifolia, Echinacea purpurea, Eucalyptus globulus essential oil, Justicia pectoralis, Magnolia officinalis, Mikania glomerata, Pelargonium sidoides, Pimpinella anisum, Salix sp, Zingiber officinale), and unknown for the rest. On the same grounds, only ibuprofen resulted promising, but we could not find compelling evidence to endorse the use of paracetamol and/or codeine. Conclusions: Our work suggests that several herbal medicines have safety margins superior to those of reference drugs and enough levels of evidence to start a clinical

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“…Although N. sativa oil concentrations and cell type used in our study were different from those of Nguyen et al, Similar results were found. However, the location of the samples, the test and other test conditions may affect the research result (26).…”

Section: Discussionmentioning

confidence: 99%

Assessment of in vitro antigenotoxic effect of nigella sativa oil

Zor¹,

Kurtoğlu²

2020

tjps

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INTRODUCTION: Cyclophosphamide is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of cyclophosphamide has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (Nigella), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of Nigella sativa oil at different concentrations against the genotoxic effects of Cyclophosphamide by micronucleus test. METHODS: For this purpose, healthy cells were treated in vitro with Nigella sativa oil at concentrations of 1, 5, 10 µg / mL and Cyclophosphamide as positive control for 68 hours. The micronuclei were then counted. RESULTS: No significant increase in micronucleus frequency was observed when the application of Nigella Sativa oil at concentrations of 1, 5, 10 µg / mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 µg / mL) compared to the Cyclophosphamide group in the groups treated with Nigella sativa oil and cyclophosphamide. DISCUSSION AND CONCLUSION: It has been shown that nigella sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of nigella sativa oil.

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In vitro toxicity, genotoxicity and antigenotoxicity of Nigella sativa extracts from different geographic locations

Cited by 14 publications

References 50 publications

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

COVID-19: Is There Evidence for the Use of Herbal Medicines as Adjuvant Symptomatic Therapy?

Assessment of in vitro antigenotoxic effect of nigella sativa oil

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