In vitro survival ofEchinococcus granulosus protoscolices in several media, at+4�C and +37�C

Casado, N.; Rodrı́guez-Caabeiro, F.; Hernández, Santiago Cortés

doi:10.1007/bf00931155

Cited by 40 publications

(27 citation statements)

References 2 publications

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“…PSC of E. granulosus were collected aseptically from hydatid cysts obtained from livers of infected cattle slaughtered in an abattoir located in the southeast of Buenos Aires province, Argentina. Vitality was assessed by muscular movements (evaluated under a light microscope), motility of flame cells, and methylene blue exclusion test (5).…”

Section: Methodsmentioning

confidence: 99%

Comparative Performances of Flubendazole and Albendazole in Cystic Echinococcosis: Ex Vivo Activity, Plasma/Cyst Disposition, and Efficacy in Infected Mice

Ceballos

Elissondo

Bruni

et al. 2011

Antimicrob Agents Chemother

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The need to identify improved therapy against cystic echinococcosis (CE) has motivated pharmacology-based research. The comparative pharmacological performances of the benzimidazole compounds flubendazole (FLBZ) and albendazole (ABZ) were addressed here. The goals of the work were as follows: (i) to evaluate the ex vivo activities of FLBZ, ABZ, and their respective metabolites against Echinococcus granulosus protoscoleces, (ii) to compare the plasma and cyst disposition kinetics for the two drugs in infected mice, and (iii) to compare the clinical efficacies of FLBZ and ABZ against CE in mice. For the ex vivo study, E. granulosus protoscoleces were incubated with FLBZ, reduced FLBZ (R-FLBZ), ABZ, and ABZ-sulfoxide (ABZSO) (10 nmol/ml). Protoscolex viability was monitored by the methylene blue exclusion test and scanning electron microscopy (SEM). For the pharmacokinetic study, BALB/c mice with CE were allocated to two different groups and orally treated with either FLBZ or ABZ (5 mg/kg of body weight), both formulated as a cyclodextrin-based solution. Blood and cyst samples were taken up to 12 h posttreatment and analyzed by high-performance liquid chromatography (HPLC). For the efficacy study, CE-infected BALB/c mice were divided into three groups: the unmedicated control group and the FLBZ-and ABZ-treated groups. Oral treatments were performed twice a day during 25 days. After treatment, all animals were killed and the weight of the cysts was recorded. Loss of protoscolex viability was observed after drug incubation. FLBZ was detected in plasma (area under the concentration-versus-time curve [AUC] ‫؍‬ 1.8 g ⅐ h/ml) and cysts (AUC ‫؍‬ 0.3 g ⅐ h/g) collected from treated infected animals. Conversely, ABZSO was the only active molecule measured in plasma (AUC ‫؍‬ 4.4 g ⅐ h/ml) and cysts (AUC ‫؍‬ 1.5 g ⅐ h/g) after ABZ treatment. FLBZ induced a 90% reduction in cyst weight in comparison to those collected from untreated control mice (P < 0.05). However, no differences in cyst weight were observed between the ABZ-treated (8.2 g) and unmedicated control (10.5 g) groups. Due to these results, we consider flubendazole to have great potential to become a drug of choice in the treatment of cystic echinococcosis.

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Section: Methodsmentioning

confidence: 99%

Comparative Performances of Flubendazole and Albendazole in Cystic Echinococcosis: Ex Vivo Activity, Plasma/Cyst Disposition, and Efficacy in Infected Mice

Ceballos

Elissondo

Bruni

et al. 2011

Antimicrob Agents Chemother

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“…Protoscoleces were removed from cysts by aseptic techniques and washed several times with PBS. Vitality was assessed using the methylene blue exclusion test (Casado et al, 1986). Samples with vitality percentages less than 60 % were discarded.…”

Section: Methodsmentioning

confidence: 99%

“…The first successful in vitro cultivation in a cystic direction was reported by Smyth (1962Smyth ( , 1967. Since then, a significant progress was made using a variety of media and conditions (Benex, 1968;Brudnjak et al, 1970;Heath & Osborn, 1976;Casado et al, 1986;Rogan & Richards, 1986;Rodriguez-Caabeiro & Casado, 1988;Casado & Rodriguez-Caabeiro, 1988a, b, 1989Denegri et al, 2002).…”

mentioning

confidence: 99%

Strain characterization ofEchinococcus granulosusprotoscoleces of cattle origin using thein vitrovesicular development

Elissondo

Dopchiz

et al. 2005

Parasite

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Summary :The aim of this work was to characterize the strain of protoscoleces of E. granulosus of cattle origin using the in vitro vesicular development. The in vitro development of these samples was compared to samples of sheep origin determined previously by genetic analyses as common sheep strain (G1). There were similarities between sheep and cattle samples not only in the time of microcysts formation, but also in the development process. Vesiculated protoscoleces and protoscoleces with posterior bladders appeared during the first week of incubation. After 14 days of culture, a laminated layer appeared like a fine membrane in one of the extremes of the protoscoleces. In the sheep samples, microcysts were observed between 19 and 20 days. In the cattle samples, microcysts appeared between 20 and 23 days. The coincidence between the development times and physiological characteristics found in the present study may indicate that the parasites from cattle and sheep were of the same strain. Résumé : CARACTÉRISATION DE SOUCHES DE PROTOSCOLEX D'ECHINOCOCCUS GRANULOSUS

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“…Cysts were classified as fertile, sterile and degenerate (calcified or caseous). Fertility was evaluated by using light microscopic observation (400X) of protoscoleces; vitality was assessed by muscular movements and motility of flame cells, and through methylene blue exclusion test [19].…”

Section: Parasitological Examinationmentioning

confidence: 99%

Retrospective study on Cystic Echinococcosis in cattle of Italy

Poglayen

Varcasia

Pipia

et al. 2017

J Infect Dev Ctries

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Introduction: Cystic Echinococcosis (CE) is one of the most widespread zoonosis of veterinary and medical importance still constituting a sanitary, economic and socio-cultural problem in Italy. Methodology: The aim of this study was to update epidemiological data on cattle CE in Italy. Data on CE positivity of 5,336 cattle were acquired from abattoir registers between January 2009 and July 2010. Morphobiological characterization of hydatids was performed by direct examination of liver and lungs of 1,664 animals butchered in the same slaughterhouses in 2010. Strain typing of parasites was carried out through the amplification and sequencing of nd1 and cox1 mitochondrial genes. Results: Overall CE prevalence was of 8.1% (430/5,336). Parasitological examination of hydatids showed an overall prevalence of 8.6% with a fertility rate of 0.7% (12/1,664). Regarding localization, hydatids were found in 8% of the livers and in 7.6% of the lungs, respectively. Among positive animals, higher prevalence was observed in the liver (93%) compared to lungs (88.1%) (p > 0.05). Conclusion: The economic loss due to organs condemnation related to CE in cattle amounted to almost € 24,000 per year in the examined abattoir during 2010. Sequence analysis showed the presence of G1 (sheep strain) or Echinococcus granulosus sensu strictu in all examined samples. The G1 confirmed, once more, its possible development into several intermediate hosts such as cattle, especially in areas like southern Italy and Sardinia where the lifecycle of the parasite is still to date carried on by sheep and dogs.

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In vitro survival ofEchinococcus granulosus protoscolices in several media, at+4�C and +37�C

Cited by 40 publications

References 2 publications

Comparative Performances of Flubendazole and Albendazole in Cystic Echinococcosis: Ex Vivo Activity, Plasma/Cyst Disposition, and Efficacy in Infected Mice

Comparative Performances of Flubendazole and Albendazole in Cystic Echinococcosis: Ex Vivo Activity, Plasma/Cyst Disposition, and Efficacy in Infected Mice

Strain characterization ofEchinococcus granulosusprotoscoleces of cattle origin using thein vitrovesicular development

Retrospective study on Cystic Echinococcosis in cattle of Italy

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