Strain characterization ofEchinococcus granulosusprotoscoleces of cattle origin using thein vitrovesicular development

Abstract

Summary :The aim of this work was to characterize the strain of protoscoleces of E. granulosus of cattle origin using the in vitro vesicular development. The in vitro development of these samples was compared to samples of sheep origin determined previously by genetic analyses as common sheep strain (G1). There were similarities between sheep and cattle samples not only in the time of microcysts formation, but also in the development process. Vesiculated protoscoleces and protoscoleces with posterior bladders appeared during the first week of incubation. After 14 days of culture, a laminated layer appeared like a fine membrane in one of the extremes of the protoscoleces. In the sheep samples, microcysts were observed between 19 and 20 days. In the cattle samples, microcysts appeared between 20 and 23 days. The coincidence between the development times and physiological characteristics found in the present study may indicate that the parasites from cattle and sheep were of the same strain. Résumé : CARACTÉRISATION DE SOUCHES DE PROTOSCOLEX D'ECHINOCOCCUS GRANULOSUS

Improvement of the In Vitro Culture of Echinococcus Granulosus Metacestodes

“…Evolution times and yield per tube of control cultures coincides with the previously reported results by our group [5] ( Table 1). The percentage of obtained cysts was around 1.6%.…”

“…granulosus protoscoleces behaviour in in vitro cultures has been quite well described previously [4][5][6][7]. A low percentage of protoscoleces develops into microcysts in in vitro systems supplemented with fetal calf serum, independently of their host origin (Table 1).…”

The effects of glucose and ascorbic acid on in vitro development of Echinococcus granulosus metacestodes

“…Moreover, these authors have cultivated protoscoleces from sheep origin in 2005, and reported that there are no differences between the ovine and bovine during the development process and at the time of cysts' formation (Elissondo et al 2005).…”