Improvement of the In Vitro Culture of Echinococcus Granulosus Metacestodes

Elissondo, Marı́a Celina

doi:10.15406/jmen.2017.04.00133

Cited by 2 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, they reported that a laminated layer appeared after 11 days of culturing under in vitro, and on day 14, some cysts with a complete laminated layer were detected. Accordingly, this was in line with our results with 6 mg/ml of glucose (Elissondo 2017). Of note, insulin is a peptide hormone that can regulate the metabolism of carbohydrate and promote the absorption of glucose; therefore, the observed similarity was expected.…”

Section: Discussionsupporting

confidence: 93%

“…The protoscoleces with the density of approximately 1800 ml were incubated in a RPMI 1640 medium (R1383, Sigma-Aldrich, USA) containing penicillin (100 IU/ml) and streptomycin (100 µg/ml), which was also supplemented with 10% (v/v) fetal bovine serum (FBS) and 4 mg/ml of D-glucose in 5% CO 2 at 37 • C (Liu et al 2013). Changes in development, including vesiculation, the appearance of laminated layer, mature cyst formation, and number and size of the cysts were all followed using an inverted microscope (Motic®, AE31, Spain) in each day for 50 days (Elissondo 2017). The medium was also changed every 3-4 days.…”

Section: The Effect Of Glucose On Development Of Egranulosus Metacestodesmentioning

confidence: 99%

“…As the cystic stage of E.granulosus develops slowly, the risk of contamination is high and because of the complication of nutritional conditions, only 1 to 11 percent of protoscoleces develop into small cysts (Elissondo 2017;Elissondo et al 2004;Rodriguez-Caabeiro and Casado 1988). Thus, in vitro culture conditions were needed to be improved.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effects of glucose and ascorbic acid on in vitro development of Echinococcus granulosus metacestodes

et al. 2021

View full text Add to dashboard Cite

Echinococcus granulosus-developed metacestodes in the cultured medium are used for the assessment of its susceptibility to different compounds; however, this procedure is time-consuming and risky. In the present study, aspirated protoscoleces from the infected sheep were used to evaluate the effects of glucose, as an energy source, as well as ascorbic acid, as an antioxidant vitamin, on larval development.Protoscoleces were maintained in RPMI 1640 culture media containing 10% fetal calf serum, as well as different concentrations of glucose (6 and 8 mg/ml) and ascorbic acid (25, 50, and 100 µg/ml). A culture medium containing 4 mg/ml of glucose was served as the control. Larger cysts were achieved in a shorter time from the medium enriched with 6 mg/ml of glucose (740 ± 20 µm) compared to the control group (420 ± 40 µm). However, in the groups treated with ascorbic acid, the number of cysts was higher in 100 µg/ml (32.5 ± 0.7) compared to the control group (12.5 ± 0.7). Additionally, the mature cysts were achieved on the 7th day of cultivation with 100 µg/ml of ascorbic acid compared to 18 days in the control group.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: The Effect Of Glucose On Development Of Egranulosus Metacestodesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of glucose and ascorbic acid on in vitro development of Echinococcus granulosus metacestodes

et al. 2021

View full text Add to dashboard Cite

show abstract

The effects of Glucose and Ascorbic acid on in vitro development of Echinococcus granulosus Metacestodes

Sajadi

Haniloo

Nadri

et al. 2021

Preprint

View full text Add to dashboard Cite

Echinococcus granulosus-developed metacestodes in the cultured medium are used for the assessment of its susceptibility to different compounds; however, this procedure is time-consuming and risky. In the present study, aspirated protoscoleces from the infected sheep were used to evaluate the effects of glucose, as an energy source, as well as ascorbic acid, as an antioxidant vitamin, on larval development. Protoscoleces were maintained in RPMI1640 culture media containing 10% fetal calf serum, as well as different concentrations of glucose (6 and 8 mg/ml) and ascorbic acid (25, 50, and 100 µg/ml). A culture medium containing 4 mg/ml of glucose was served as the control. Larger cysts were achieved in a shorter time from the medium enriched with 6 mg/ml of glucose (740 ± 20 µm) compared to the control group (420 ± 40 µm). However, in the groups treated with ascorbic acid, the number of cysts was higher in 100 µg/ml (32.5 ± 0.7) compared to the control group (12.5 ± 0.7). Additionally, the mature cysts were achieved on the 7th day of cultivation with 100 µg/ml of ascorbic acid compared to 18 days in the control group.

show abstract

Improvement of the In Vitro Culture of Echinococcus Granulosus Metacestodes

Cited by 2 publications

References 13 publications

The effects of glucose and ascorbic acid on in vitro development of Echinococcus granulosus metacestodes

The effects of glucose and ascorbic acid on in vitro development of Echinococcus granulosus metacestodes

The effects of Glucose and Ascorbic acid on in vitro development of Echinococcus granulosus Metacestodes

Contact Info

Product

Resources

About