In vitro study on immune response modifiers as novel medical treatment options for cholesteatoma

Uzun, Tankut; Çaklı, Hamdi; Coşan, Didem Turgut; İncesulu, Şaziye Armağan; Kaya, Ercan; Yıldız, Erdost

doi:10.1016/j.ijporl.2021.110743

Cited by 4 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main anti-inflammatory cytokine, Interleukin-10 (IL-10), on the other hand, works against high pro-inflammatory cytokine levels in cholesteatoma tissue [17,18]. An imbalance of IL-1 and IL-10 is believed to cause an uncontrolled inflammatory process, which is destructive for the surrounding bone in the middle ear [20].…”

Section: Introductionmentioning

confidence: 99%

Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma

2021

View full text Add to dashboard Cite

The aim of this study was to describe the appearance and distribution of tissue remodeling markers (MMP-2, MMP-9, TIMP-2, TIMP-4), Sonic hedgehog gene protein (Shh), pro- and anti-inflammatory cytokines (IL–1, IL–10), transcription factor (NF-κβ), proliferation marker (Ki–67), angiogenetic factor (VEGF), tissue defensins (HβD–2, HβD–4) of the pediatric cholesteatoma. Sixteen cholesteatoma samples were obtained from children, eleven skin controls from cadavers. Tissues were stained for MMP-2, MMP-9, TIMP-2, TIMP-4, Shh, IL–1, IL–10, NF-κβ, Ki–67, VEGF, HβD–2, HβD–4. Non-parametric statistic, Mann–Whitney, and Spearman’s coefficient was used. A statistically significant difference was seen between Shh and HβD–2 in perimatrix and control connective tissue, between NF-κβ in cholesteatoma and control skin, and between HβD–4 in matrix and skin epithelium. Complex intercorrelations between MMPs, NF-κβ and VEGF cause the intensification of angiogenesis in cholesteatoma. The persistent increase in Shh gene protein expression in cholesteatoma perimatrix suggests the stimulation of the cholesteatoma growth in children. Similar expression of IL-1 and IL-10 and their intercorrelation, proves there is a balance between pro- and anti-inflammatory cytokines. NF-κβ, and not Ki-67, seems to be the main inducer of cellular proliferation. The main antimicrobial protection is provided by HβD-2.

show abstract

Section: Introductionmentioning

confidence: 99%

Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma

2021

View full text Add to dashboard Cite

show abstract

“…By contrast, interleukin 10 is the most active anti-inflammatory cytokine, and its impaired expression can exacerbate the inflammatory response and lead to tissue damage [ 21 ]. The disproportion between IL-1 and IL-10 in cholesteatoma could be one of many causes of its aggressiveness [ 22 ]. Furthermore, human beta defensins 2 and 4 reduce the inflammation caused selectively by Ps.…”

Section: Introductionmentioning

confidence: 99%

Morphopathogenesis of Adult Acquired Cholesteatoma

2023

View full text Add to dashboard Cite

Background and Objectives. The aim of this study was to compare the distribution of proliferation markers (Ki-67, NF-κβ), tissue-remodeling factors (MMP-2, MMP-9, TIMP-2, TIMP-4), vascular endothelial growth factor (VEGF), interleukins (IL-1 and IL-10), human beta defensins (HβD-2 and HβD-4) and Sonic hedgehog gene protein in cholesteatoma and control skin. Methods. Nineteen patient cholesteatoma tissues and seven control skin materials from cadavers were included in the study and stained immunohistochemically. Results. Statistically discernible differences were found between the following: the Ki-67 in the matrix and the Ki-67 in the skin epithelium (p = 0.000); the Ki-67 in the perimatrix and the Ki-67 in the connective tissue (p = 0.010); the NF-κβ in the cholesteatoma matrix and the NF-κβ in the epithelium (p = 0.001); the MMP-9 in the matrix and the MMP-9 in the epithelium (p = 0.008); the HβD-2 in the perimatrix and the HβD-2 in the connective tissue (p = 0.004); and the Shh in the cholesteatoma’s perimatrix and the Shh in the skin’s connective tissue (p = 0.000). Conclusion. The elevation of Ki-67 and NF-κβ suggests the induction of cellular proliferation in the cholesteatoma. Intercorrelations between VEGF, NF-κβ and TIMP-2 induce neo-angiogenesis in adult cholesteatoma. The similarity in the expression of IL-1 and IL-10 suggests the dysregulation of the local immune status in cholesteatoma. The overexpression of the Sonic hedgehog gene protein in the cholesteatoma proves the selective local stimulation of perimatrix development.

show abstract

Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma

2023

Int Adv Otol

View full text Add to dashboard Cite

Background: This study aimed to examine the roles of miR-10a-5p and phosphatidylinositol-4,5-bisphosphonate 3-kinase catalytic subunit α in the pathogenesis of middle ear cholesteatoma. Methods: We enrolled 27 patients with middle ear cholesteatoma and collected samples of intraoperative cholesteatoma and normal posterior ear skin tissues. The mRNA expression levels of miR-10a-5p and PIK3CA were detected using real-time quantitative polymerase chain reaction. PIK3CA protein expression was measured by immunohistochemistry and western blotting. Results: Middle ear cholesteatoma tissues showed significantly lower miR-10a-5p expression levels and significantly higher PIK3CA expression levels than normal posterior ear skin tissues (both P < .05). Furthermore, the miR-10a-5p and PIK3CA expression levels were significantly negatively correlated in middle ear cholesteatoma tissues ( r = −0.926, P < .001). Conclusion: Low miR-10a-5p expression levels in middle ear cholesteatoma tissues may inhibit the growth and proliferation of cholesteatoma, whereas high PIK3CA expression level may promote its growth and proliferation. In addition, miR-10a-5p may affect the proliferation and differentiation of cholesteatoma by negatively regulating its target gene, PIK3CA .

show abstract

In vitro study on immune response modifiers as novel medical treatment options for cholesteatoma

Cited by 4 publications

References 58 publications

Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma

Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma

Morphopathogenesis of Adult Acquired Cholesteatoma

Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma

Contact Info

Product

Resources

About