Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma

Abstract

Background: This study aimed to examine the roles of miR-10a-5p and phosphatidylinositol-4,5-bisphosphonate 3-kinase catalytic subunit α in the pathogenesis of middle ear cholesteatoma. Methods: We enrolled 27 patients with middle ear cholesteatoma and collected samples of intraoperative cholesteatoma and normal posterior ear skin tissues. The mRNA expression levels of miR-10a-5p and PIK3CA were detected using real-time quantitative polymerase chain reaction. PIK3CA protein expression was measured by immunohistochemistry and western blotting. Results: Middle ear cholesteatoma tissues showed significantly lower miR-10a-5p expression levels and significantly higher PIK3CA expression levels than normal posterior ear skin tissues (both P < .05). Furthermore, the miR-10a-5p and PIK3CA expression levels were significantly negatively correlated in middle ear cholesteatoma tissues ( r = −0.926, P < .001). Conclusion: Low miR-10a-5p expression levels in middle ear cholesteatoma tissues may inhibit the growth and proliferation of cholesteatoma, whereas high PIK3CA expression level may promote its growth and proliferation. In addition, miR-10a-5p may affect the proliferation and differentiation of cholesteatoma by negatively regulating its target gene, PIK3CA .

Expression and Regulatory Mechanisms of MicroRNA in Cholesteatoma: A Systematic Review

“…In total, 118 articles were retrieved through the database search. After applying the search strategy described above, a total of 18 articles were identified that met all inclusion criteria [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47]. A flow diagram of the detailed study selection process is presented in Figure 1.…”

“…In another study [41], middle ear cholesteatoma tissues showed significantly decreased miR-10a-5p expression and increased PIK3CA expression compared to normal posterior ear skin tissues (p < 0.05). The results of target gene prediction revealed that PIK3CA may be an miR-10a-5p target gene.…”

“…Retroauricular skin has been used as a control material in many of the included studies [31,33,[35][36][37][38][40][41][42][43][44][45]47]. There are reports that the selection of control group material in cholesteatoma studies can be important for the outcome of the studies, with deep meatal skin specimens being more similar in protein profile to cholesteatoma than retroauricular skin [165,166].…”