In Vitro Studies on the Relationship Between the Antioxidant Activities of Some Berry Extracts and Their Binding Properties to Serum Albumin

Abstract

The aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays and mass spectrometry. The total phenolic content was the highest (P < 0.05) in water extract of blueberries (46.6 ± 4.2 mg GAE/g DW). The highest antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and Cupric reducing antioxidant capacity were in water extracts of blueberries, showing 108.1 ± 7.2 and 131.1 ± 9.6 μMTE/g DW with correlation coefficients of 0.9918 and 0.9925, and by β-carotene linoleate assay at 80.1 ± 6.6 % with correlation coefficient of 0.9909, respectively. The water extracts of berries exhibited high binding properties with human serum albumin in comparison with quercetin. In conclusion, the bioactive compounds from a relatively new source of gooseberries in comparison with blueberries and cranberries have the potential as food supplementation for human health. The antioxidant and binding activities of berries depend on their bioactive compounds.

LC–MS/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes

“…Also, restricting the use of synthetic antioxidants leaded to an incremental interest of natural antioxidant sources. Therefore, there is an increasing way in consumer priorities based on natural antioxidants, which have given a driving force to the initiatives to detect many new sources of antioxidants (Dymerski et al, 2015;Gorinstein et al, 2013;Gülç in, 2008aGülç in, , 2009Namiesnik et al, 2014).…”

Effect of Calcium Chloride Treatments on Quality Characteristics of Blackberry, Raspberry and Strawberry Fruits After Cold Storage

“…Berries are excellent sources of phytochemicals that are believed to have significant biological activity. Berry containing elevated levels of bioactive compounds (Skrovankova et al, 2015;Halvorsen et al, 2002;De Souza et al, 2014;Slatnar et al, 2012;Namiesnik et al, 2014;Diaconeasa et al, 2015), are attracting considerable attention due to their potential to lower the risk of chronic diseases and their associated huge healthcare costs (Beattie et al, 2005;Karlund et al, 2014;Pantelidis et al, 2007). Phenolics may contribute to this protective effect.…”

Fluorescence studies by quenching and protein unfolding on the interaction of bioactive compounds in water extracts of kiwi fruit cultivars with human serum albumin

“…These results were in direct relationship with the antioxidant properties of the extracts. Our very recent results [25,26] showed that the fluorescence is significantly quenched, because of the formation of complexes between proteins and phenolic acids and flavonoids. This interaction was investigated using tryptophan fluorescence quenching.…”

“…It illustrates the influence of last two mentioned effects on the monitored characteristics from global point of view. Based on fluorescence profiles which exhibited high binding properties with HSA the seven new kiwi fruit cultivars were classified for three groups: 'Hayward' (including 'Daheung', 'Haenam', Hwamei' and 'SKK12'), 'Bidan' and 'Hort 16A'[9,10,25,26]. The properties of kiwi fruit's phenol extracts showed their ability to quench HSA, forming the complexes similar to the ones between the proteins and pure…”