In Vitro Steroidogenesis on Oocyte Development in the Starry Flounder, Platichthys stellatus

Abstract

In this study, oocyte steroidogenesis are investigated in relation to oocyte development in the starry flounder, Platichthys stellatus, a marine multiple spawner. Vitellogenic (0.52 and 0.55 mm oocyte diameter) and mature oocytes (0.63, 0.66 and 0.71 mm oocyte diameter) were incubated in vitro in the presence of [3H]17α-hydevrepoxyprogesterone ([3H]17α-OHP) as a precursor. Steroid metabolites were extracted from the incubated media and oocytes, the extracts were separated and identified by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The major metabolites produced from [3H]17α-OHP were andevrepogens [andevrepostenedione (A4) and testosterone (T)] and estrogens [17β-estradiol (E2) and estrone (E1)] and progestins [17α,20α-dihydevrepoxy-4-pregnen-3-one (17α20αP) and 17α,20β-dihydevrepoxy-4-pregnen-3-one (17α20βP)] in vitellogenic and mature oocytes. The results from this study suggest the potential roles of E1 in the oocytes with diameter 0.52-0.71 mm, 17α20αP and 17α20βP at the oocytes of 0.63, 0.66 and 0.71 mm.

In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation

“…At the end of the incubation, steroids extraction, separation and identification of metabolites were followed by our previous studies ( Hwang et al, 2012 ; Baek et al, 2013 ; Lee & Baek, 2015 ). Steroid metabolites produced from [ 3 H]-17 α P were compared with the values were expressed as photo-stimulated luminescence (PSL) – background (BG) per mm 2 of each fraction area from autoradiography.…”

Steroid Metabolism in the Blackfin Flounder Glyptocephalus stelleri during Oocyte Maturation

“…(starry flounder) (Baek et al, 2013). Canario and Scott (1990Scott ( , 1991 17 20 P marine flatfish Limanda limanda MIS .…”