Steroid Metabolism in the Blackfin Flounder Glyptocephalus stelleri during Oocyte Maturation

Lee, Hae Won; Baek, Hea Ja

doi:10.5657/kfas.2015.0483

Cited by 1 publication

(2 citation statements)

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“…Among the metabolites, A 4 was predominant in all size of oocytes even in the GVM stage (500 μm oocyte diameter). In our previous study, A 4 was also produced dominantly in vitellogenic oocytes from red lip mullet, Chelon haematocheilus, blacktip grouper, Epinephelus fasciatus, and blackfin flounder, Glyptocephalus stelleri (Baek et al, 2011;Hwang et al, 2012;Lee & Baek, 2015). A few studies reported that A 4 is related to sex change in protogynous hermaphrodite, Monopterus albus and Sparisoma viride (Yeung & Chan, 1987;Cardwell & Liley, 1991;Yeung et al, 1993).…”

Section: Fig 2 Autoradiograms Of Steroid Metabolites Produced From [ 3 H]17α-hydroxyprogesterone After 24 Hmentioning

confidence: 92%

“…At the end of the incubation, steroids extraction, separation and identification of metabolites were followed by our previous studies ( Hwang et al, 2012 ; Baek et al, 2013 ; Lee & Baek, 2015 ). Steroid metabolites produced from [ 3 H]-17 α P were compared with the values were expressed as photo-stimulated luminescence (PSL) – background (BG) per mm 2 of each fraction area from autoradiography.…”

Section: Methodsmentioning

confidence: 99%

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In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation

Hwang¹,

Baek²

2021

Dev. Reprod.

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We studied steroid metabolites produced from red-spotted grouper ovarian follicles during maturation. Oocytes with 350-500 μm diameter were in vitro incubated in the presence of [ 3 H] 17α-hydroxyprogesterone as a precursor. Steroid metabolites were extracted from incubated media and oocytes. The extracts were separated and identified using thin layer chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry. The identified metabolites were androstenedione (A 4 ), testosterone (T) and estrone (E 1 ). The metabolites of A 4 was dominant in all size of oocytes and it was the highest in 480 μm diameter oocytes. The metabolites of two progestins, 17α,20β-dihydroxy-4pregnen-3-one and 17α,20α-dihydroxy-4-pregnen-3-one were detected in the oocytes less than 480 μm diameter although they were not identified definitely. In the oocytes of 480 μm diameter, metabolite of progestin was the highest, and germinal vesicle (GV) was still in the middle of cytoplasm. In the oocytes of 500 μm diameter, GV was began to migrate and the major metabolites were A 4 and E 1 . The metabolite of E 1 was detected in all size of oocytes and it was higher than that of E 2 . These results suggest that oocytes of 480 μm diameter are the transitional stage involving steroidogenic shift to final oocyte maturation and potential function of E 1 during maturation process.

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Section: Fig 2 Autoradiograms Of Steroid Metabolites Produced From [ 3 H]17α-hydroxyprogesterone After 24 Hmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation

Hwang¹,

Baek²

2021

Dev. Reprod.

Self Cite

View full text Add to dashboard Cite

show abstract

Steroid Metabolism in the Blackfin Flounder Glyptocephalus stelleri during Oocyte Maturation

Cited by 1 publication

References 14 publications

In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation

In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation

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