This study was conducted to determine the stress response [ethological (operculum movement number (OMN)), hematological (hematocrit and hemoglobin), biochemical (glucose, cortisol and glutamic oxaloacetic transaminase (GOT))] in red spotted grouper, Epinephelus akaara during exposure of different water temperature in winter season. This species (Total length, 18.56±0.34 cm) previously maintained in water temperature of 15°C were transferred to 15, 20 and 25°C. During experimental period (7 days), OMN, hematocrit (Ht), glucose and GOT values were significantly high in 15°C when compared to 20 and 25°C. Hemoglobin value was also increased at 15°C , but no significant differences. There was no differences in cortisol levels among the temperature groups. No fish mortality was observed during the experimental period. From these results, 15°C is likely more stressful to red spotted grouper than 20°C and 25°C. These observations confirm that red spotted grouper adapts better to temperatures between 20 and 25°C during the winter season.
This study focused on the association of prostaglandins and a progestin,
17α, 20β-dihydroxy-4-pregnen-3-one (17α20βP) during
the ovulation process in longchin goby,
Chasmichthys
dolichognathus
. We performed several
in vitro
experiments using 850–920 μm diameter oocytes which were at the
migratory nucleus stage. With the 890–920 μm diameter oocytes, no
significant difference in ovulation was observed in any of the prostaglandins
(PGE1, PGE2, and PGF2α) treated groups although PGE2 and PGF2α at
concentrations of 50 ng/mL increased ovulation slightly compared with controls;
however, 17α20βP production was stimulated with PGE1 alone at low
concentrations (5 ng/mL). In 850 μm diameter oocytes, PGF2α at
concentrations of 50 and 500 ng/ml resulted in a significant increase in
ovulation. 17α20βP (50 ng/ml) alone had no observable effect on
ovulation, but in the combined of PGF2α 50 or 500 ng/ml it caused the
greatest effect on ovulation. The sensitivity of oocytes to the induction of
ovulation varies between 850 and 890–920 μm, it appeared to vary
depending on the migration status of nucleus. These results suggest that
PGF2α (or combined of 17α20βP) was more potent in inducing
ovulation of the longchin goby.
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