In Vitro Simultaneous Transfer of Lipids to HDL in Coronary Artery Disease and in Statin Treatment

Prete, Ana Cristina Lo; Dina, C.H.; Azevedo, Carolina Heitmann Mares; Puk, Camila Góes; Lopes, Neuza; Hueb, Whady; Maranhão, Raul C.

doi:10.1007/s11745-009-3342-2

Cited by 58 publications

(75 citation statements)

References 39 publications

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“…PON1 activity is substantially lower in patients with CAD, ACS, CKD, and RA, whereas lipoprotein-associated phospholipase A 2 (LpPLA 2 ) activity is increased in CKD and psoriasis (89,137,160,165,(171)(172)(173)(174)(175). Dysregulating the activity of PON1 and LpPLA 2 in this fashion not only compromises the antioxidative activity of HDL, but also promotes the formation of atherosclerotic plaques.…”

Section: Composition and Role Of Hdl-associated Proteinsmentioning

confidence: 99%

The Changing Face of HDL and the Best Way to Measure It

Karathanasis¹,

Freeman

Gordon

et al. 2017

Clinical Chemistry

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BACKGROUND: HDL cholesterol (HDL-C) is a commonly used lipid biomarker for assessing cardiovascular health. While a central focus has been placed on the role of HDL in the reverse cholesterol transport (RCT) process, our appreciation for the other cardioprotective properties of HDL continues to expand with further investigation into the structure and function of HDL and its specific subfractions. The development of novel assays is empowering the research community to assess different aspects of HDL function, which at some point may evolve into new diagnostic tests. CONTENT:This review discusses our current understanding of the formation and maturation of HDL particles via RCT, as well as the newly recognized roles of HDL outside RCT. The antioxidative, antiinflammatory, antiapoptotic, antithrombotic, antiinfective, and vasoprotective effects of HDL are all discussed, as are the related methodologies for assessing these different aspects of HDL function. We elaborate on the importance of protein and lipid composition of HDL in health and disease and highlight potential new diagnostic assays based on these parameters.

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Section: Composition and Role Of Hdl-associated Proteinsmentioning

confidence: 99%

The Changing Face of HDL and the Best Way to Measure It

Karathanasis¹,

Freeman

Gordon

et al. 2017

Clinical Chemistry

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“…The transfer rates of cholesteryl ester, phospholipid, free cholesterol and triglyceride from cholesterol-rich emulsions to HDL was determined in the Lipid Metabolism Laboratory of the Heart Institute (InCor) as previously described (Daminelli et al, 2008;Giribela et al, 2009;Lo Prete et al, 2009;Puk et al, 2009). Lipid nanoemulsions were prepared from a lipid mixture of 40 mg cholesteryl oleate, 20 mg egg phosphatidylcholine, 1 mg triolein and 0.5 mg cholesterol purchased from the Sigma Chemical Company (St Louis, MO, USA).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…containing no precipitation reagents (Daminelli et al, 2008;Lo Prete et al, 2009). Intra-assay precision studies included 10 replicates of samples.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Effects of margarines and butter consumption on lipid profiles, inflammation markers and lipid transfer to HDL particles in free-living subjects with the metabolic syndrome

et al. 2010

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Objective: Our purpose was to examine the effects of daily servings of butter, no-trans-fat margarine and plant sterol margarine, within recommended amounts, on plasma lipids, apolipoproteins (Apos), biomarkers of inflammation and endothelial dysfunction, and on the transfer of lipids to HDL particles in free-living subjects with the metabolic syndrome. Methods: This was a randomized, single-blind study where 53 metabolic syndrome subjects (62% women, mean age 54 years) received isocaloric servings of butter, no-trans-fat margarine or plant sterol margarine in addition to their usual diets for 5 weeks. The main outcome measures were plasma lipids, Apo, inflammatory and endothelial dysfunction markers (CRP, IL-6, CD40L or E-selectin), small dense LDL cholesterol concentrations and in vitro radioactive lipid transfer from cholesterol-rich emulsions to HDL. Difference among groups was evaluated by analysis of variance. Results: There was a significant reduction in Apo-B (À10.4 %, P ¼ 0.043) and in the Apo-B/Apo-A-1 ratio (À11.1%, P ¼ 0.034) with plant sterol margarine. No changes in plasma lipids were noticed with butter and no-trans-fat margarine. Transfer rates of lipids to HDL were reduced in the no-trans-fat margarine group: triglycerides À42.0%, (Po0.001 vs butter and sterol margarine) and free cholesterol À16.2% (P ¼ 0.006 vs sterol margarine). No significant effects were noted on the concentrations of inflammatory and endothelial dysfunction markers among the groups. Conclusions: In free-living subjects with the metabolic syndrome consumption of plant sterol and no-trans-fat margarines within recommended amounts reduced, respectively, Apo-B concentrations and the ability of HDL to accept lipids.

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“…ferent (p < 0.05) when compared to normolipidemic group. The fact that the participating individuals did not use lipid-lowering drugs at the time of sampling was important to exclude treatment interference on lipid metabolism, a really important aspect not found in several important published studies [18] [19].…”

Section: Discussionmentioning

confidence: 99%

Atherogenic Indices and HDL Particle Size as Laboratory Parameters to Evaluate Cardiovascular Risk in the Presence of Dyslipidemia

Santos¹,

Vieira²,

Deus³

et al. 2014

JBPC

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Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remodeling. Calculated indices and evaluation of lipoprotein particle size have widely been used to predict cardiovascular risk. The aim of this study was to evaluate HDL particle size and LDL particle size estimate based on TG/HDL-C as well as apoB/apoA-I ratio as possible marker and atherogenic indices, respectively, of cardiovascular disease risk in the presence of dyslipidemia. We evaluated 100 individuals of both gender, without treatment with lipid-lowering drugs, 27 normolipidemic and 73 dyslipidemic, such as isolated hypercholesterolemia (n = 16), isolated A. P. C. Santos et al. 25hypertriglyceridemia (n = 17), low HDL-C (n = 26) and mixed dyslipidemia (n = 14). The HDL particle size did not differ between groups. The TG/HDL-C ratio was higher in groups with isolated hypertriglyceridemia (4.2 ± 1.5), low HDL-C (5.2 ± 3.1) and mixed dyslipidemia (5.3 ± 1.6). The apoB/apoA-I ratio was increased in all groups of dyslipidemia (apoB/apoA-I > 0.5) when compared to normolipidemic (apoB/apoA-I = 0.5, p < 0.001). There was a positive linear correlation between the TG/HDL-C ratio and the apoB/apoA-I ratio in low HDL-C group (r = 0.507, p = 0.008, Spearman). The results suggest that the evaluations of lipoproteins particles remodeling markers and the use of calculated indices may contribute to the evaluation of cardiovascular disease risk when dyslipidemia take place.

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In Vitro Simultaneous Transfer of Lipids to HDL in Coronary Artery Disease and in Statin Treatment

Cited by 58 publications

References 39 publications

The Changing Face of HDL and the Best Way to Measure It

The Changing Face of HDL and the Best Way to Measure It

Effects of margarines and butter consumption on lipid profiles, inflammation markers and lipid transfer to HDL particles in free-living subjects with the metabolic syndrome

Atherogenic Indices and HDL Particle Size as Laboratory Parameters to Evaluate Cardiovascular Risk in the Presence of Dyslipidemia

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