In vitro screening of ovarian tumor specific peptides from a phage display peptide library

Abstract

To develop more biomarkers for diagnosis and therapy of ovarian cancer, a 12-mer phage display library was used to isolate peptides that bound specifically to the human ovarian tumor cell line SK-OV-3. After five rounds of in vitro screening, the recovery rate of phages showed a 69-fold increase over the first round of washings and a group of phage clones capable of binding to SK-OV-3 cells were obtained. A phage clone named Z1 with high affinity and specificity to SK-OV-3 cells was identified in vitro. More importantly, the synthetic biotin-labeled peptide, ZP1 (=SVSVGMKPSPRP), which corresponded to the sequence of the inserted fragment of Z1, demonstrated a high specificity to SK-OV-3 cells especially when compared to other cell lines (A2780 and 3T3). ZP1 might therefore be a biomarker for targeting drug delivery in ovarian cancer therapy.

Subtype-specific binding peptides enhance the therapeutic efficacy of nanomedicine in the treatment of ovarian cancer

“…Several reports have been published on the development of targeting peptides with favorable tumor cellspecific binding and internalization in the treatment of ovarian cancer. However, further descriptions of the therapeutic efficacy of these developing peptides are not available [36][37][38].…”

A synthetic peptide inhibits human ovarian cancer cell motility

“…Immunouorescent staining showed that the biotin-SV-peptide binds to the surface of SK-OV-3, A549, and HUVEC cells but is internalized by A2780 cells (Fig. 22 The apparent discrepancy in the relative affinities to the ovarian cancer cell lines (SK-OV-3 and A2780) may be related to assay conditions that could detach more loosely bound abundant peptides. Fluorescence intensity was ranked in the order of tumor cells (A2780, A549 and SK-OV-3) > HUVEC (Fig.…”

“…22 The high specicity of the SV-peptide for SK-OV-3 cell line suggests that it can be used as a biomarker for targeting drug delivery and as a drug for tumor therapy. 22 The high specicity of the SV-peptide for SK-OV-3 cell line suggests that it can be used as a biomarker for targeting drug delivery and as a drug for tumor therapy.…”

Combinatorial Peptide Libraries: Mining for Cell-Binding Peptides

“…This peptide was conjugated to ~100 nm sized PEG–poly(lactic- co -glycolic acid) (PLGA) nanoparticles loaded with the near-infrared dye DiR. 206 After tail vein injection into mice, these particles homed to the brain as evidenced by live animal fluorescence imaging. Ex vivo imaging of organs after the mice were sacrificed revealed that control nanoparticles without peptide primarily accumulated in the liver and spleen and did not accumulate in the brain.…”