In vitro Screening of Asteraceae Plant Species Against Trypanosoma cruzi

Screening of Southeastern BrazilianMikania. Species onTrypanosoma cruzi.

“…In the research about the trypanocidal effects of plants, we decided to verify the trypanocidal activity of M.hoehnei, M. stipulacea, M. cordifolia, M. camporum, M. lasiandrae, and M. micrantha, considering that extracts as well as pure compounds obtained from the Asteraceae family have been reported to possess significant trypanocidal activity (Chiari et al, 1996;Muelas-Serrano et al, 2000;Taleb-Contini et al, 2004). In this study, the trypanocidal activity of 14 extracts of plants of the Mikania genus was evaluated against the trypomastigote forms of T. cruzi Y strain.…”

“…The species M. cordifolia had already been tested by Chiari et al (1996), Muelas-Serrano et al (2000), and Rojas de Arias et al (1995), however, different solvents and concentrations were used.…”

“…For instance, Rojas de Arias et al (1995), Chiari et al (1996), Muelas-Serrano et al (2000), and Taleb-Contini et al (2004) demonstrated that species belonging to the Asteraceae family have potential trypanocidal activity. The genus Mikania is the largest genus in the tribe Eupatorieae (Asteraceae), comprising nearly 450 species, which go under the name ''guaco'' (Herz, 1998).…”

Biological activity of the aqueous extract of Lychnophora pinaster Mart.

“…The assay was performed according Chiari et al (1996): Albino mice infected with the Y strain of T. cruzi were used to provide trypomastigotes. The lyophilized extract was suspended in dimethyl sulfoxide (DMSO) (0.2 mL) plus Krebs-Ringer-glucose (1.8 mL) and mixed with infected blood (0.2 mL).…”

“…Such activity was due to the fl avonoid quercetin-7,3',4'-trimethyl ether and lychnopholic acid (Jordão et al, 2004). Aerial parts ethanol extract from L. villosissima and isolated 15-deoxygoyasenzolide (Chiari et al, 1991), L. passerina ethanol extract and isolated goyazensolide and L. trichocarpha and isolated lychnopholide and eremantholide showed in vitro trypanocidal activity against trypomastigote forms of T. cruzi Chiari et al, 1996). The ethyl acetate extract from the aerial parts of L. granmongolense also exhibited trypanocidal (trypomastigote forms of T. cruzi) activity .…”