In vitro regeneration of Persian poppy (Papaver bracteatum)

Abstract

Persian poppy (Papaver bracteatum Lindl.) is an important commercial source of medicinal opiates and related compounds. In this research, calli were induced from seeds, roots, cotyledons and hypocotyls of P. bracteatum at a high efficiency. The optimized callus induction media consisted of the Murashige and Skoog (MS) basic media supplemented with 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin and 15 mg/L ascorbic acid. The concentrations of 2,4-D and ascorbic acid were found critical to callus induction and proliferation. Subsequent subcultures resulted in excellent callus proliferation. Ascorbic acid at concentration 15 mg/L increased the callus proliferation significantly. Maximum callus growth was achieved when the explants were incubated at 25• C. MS salts at full strength were found inhibitory for callus induction, while ľ MS salts were found to favor callus induction. Shoot regeneration of calli in vitro was achieved on ľ MS medium containing 0.5 mg/L benzylamine purine and 1.0 mg/L naphthalene acetic acid. Analysis of alkaloid extracts from Persian poppy tissues by high-performance liquid chromatography showed that thebaine accumulated in the tissues of plants. The thebaine alkaloid profile of the Persian poppy is a well-defined model to evaluate the potential for metabolic engineering of thebaine production in P. bracteatum.

Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production

“…Therefore, the addition of 15 mg/L ascorbic acid to the medium was effective in reducing browning of explants and calli of P. bracteatum. Similar behavior has been previously reported in P. bracteatum (Rostampour et al, 2010).…”

High frequency somatic embryogenesis, regeneration and correlation of alkaloid biosynthesis with gene expression in Papaver somniferum

“…We also Seeds from sterilized capsule observed that half strength MS was inhibitory for callus induction, while full strength MS was found to be favorable in callus induction. Similar results were observed by other groups working on poppy (Rostampour et al 2010). …”

“…The in vitro regeneration in majority of alkaloid accumulating plants is a challenging task and highly dependent on genotype (Rostampour et al 2009(Rostampour et al , 2010. However, an efficient plant regeneration system is prerequisite for development of transformation system of these plants with objectives related to pathway engineering through manipulation of alkaloid biosynthetic genes.…”

Plant Tissue Cultures of Juniperus virginiana

“…To find the optimal concentration of auxins and cytokinins for the proliferation of the callus culture, various combinations of 2,4-dichlorophenoxy-acetic acid (2,4-D), α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), kinetin and benzylaminopurine (BAP) were added to the media [10][11][12]. To reduce the browning of the induced calli, ascorbic acid (15 mg/L, 20 mg/L, 30 mg/L and 200 mg/L) was added to the medium as an antioxidant [13,14].…”