Plant Tissue Cultures of <i>Juniperus virginiana</i>

Kašparová, Markéta; Spilková, Jiřina; Cvak, Ladislav; Siatka, T; Martin, Jan

doi:10.1177/1934578x1601100533

Cited by 3 publications

(3 citation statements)

References 10 publications

(18 reference statements)

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“…However, after ten subcultures, the callus lost its ability for multiplication. In turn, callus cultures of J. virginiana were induced using fresh leaves of three different varieties of this tree as explants [57]. The highest callus induction frequency was 88.1% for the variety 'Glauca' on SH medium containing 3.0 mg L −1 NAA and 0.2 mg L −1 KIN.…”

Section: Callus Induction From Different Explantsmentioning

confidence: 99%

Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Hazubska-Przybył

2019

Forests

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The genus Juniperus (of the Cupressaceae family) is the second most prevalent group of conifers on Earth. Juniper species are widely dispersed in the Northern Hemisphere, in Europe and Asia, and in Africa and Central America. Juniper species are resistant to dry climates and can adapt to difficult environmental conditions. Most juniper species are important in both ecological and economic terms. However, today, many forests in which junipers occur are being reduced in size due to both natural causes (fires, for example) and human activity (uncontrolled exploitation of forests, etc.). Also, climate changes may have adversely affected the range of populations of different juniper species. For this reason, some juniper species are now categorized as rare or endangered, and require immediate protective action. Therefore, there is an urgent need to develop effective strategies for ex situ conservation, including reliable procedures for Juniperus sp. reproduction for future reintroduction and restoration programs. The conservation strategies used until now with traditional forestry techniques (seed propagation, rooted cuttings, grafting) have not been satisfactory in many cases. Thus, increasing attention is being paid to the possibilities offered by in vitro culture technology, which enables the conservation and mass clonal propagation of different coniferous tree species. In this mini-review, we summarize the current state of knowledge regarding the use of various methods of the propagation of selected Juniperus species, with a particular emphasis on in vitro culture techniques.

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Section: Callus Induction From Different Explantsmentioning

confidence: 99%

Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Hazubska-Przybył

2019

Forests

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“…The sub-cultivation interval lasted for 21 days. [16] Elicitation: On the 14 th day of cultivation (in the mid-exponential growth phase) [16], the suspension cultures were treated with cinnamic acid, jasmonic acid and salicylic acid. One mL of an ethanolic cinnamic acid solution (in concentrations of 0.1 mmol/L, 1 mmol/L, 10 mmol/L and 100 mmol/L), 1 mL of an ethanolic jasmonic acid solution (in concentrations of 0.005 mmol/L, 0.05 mmol/L, 0.5 mmol/L and 5 mmol/L) and 1 mL of an aqueous salicylic acid solution (in concentrations of 0.01 mmol/L, 0.1 mmol/L, 1 mmol/L and 10 mmol/L) were added to the SH medium (30 mL).…”

Section: Juniperus Virginiana Suspension Culturesmentioning

confidence: 99%

Effect of Precursor and Phytohormones on Podophyllotoxin Production in Juniperus virginiana Suspension Cultures

Kašparová

Pilařová

Tůmová

et al. 2018

Natural Product Communications

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Our results showed that cinnamic acid can increase podophyllotoxin production in Juniperus virginiana suspension cultures. The best effect was manifested after a 24-hours application of a 10 mmol/L concentration. The highest podophyllotoxin content was determined at 1.47 mg/g DW and the production was statistically significantly stimulated by about 444% in comparison with the control. Comparison of podophyllotoxin production in the cinnamic acid- and salicylic acid-elicited J. virginiana suspension cultures confirms that the maximum increase in both cases was induced by the 24-hours application of the 10 mmol/L concentration. In contrast, the best effect of jasmonic acid was manifested after the longest 168-hours application of a 5 mmol/L concentration.

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“…J. virginiana suspension cultures (varieties 'Hetzii', 'Glauca', 'Grey Owl') were derived from twoyears-old callus cultures on the same medium and under the same conditions as callus cultures. The callus and suspension cultures of the variety 'Glauca' had the best viability and thus provided the most biomass [8].…”

mentioning

confidence: 99%

Production of Podophyllotoxin by Plant Tissue Cultures of Juniperus virginiana

Kašparová

Martina

Tůmová

et al. 2017

Natural Product Communications

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Plant tissue cultures are a potential source of secondary metabolites. However, their production, when compared with intact plants, is usually lower. Phenylalanine, a biogenetic precursor of podophyllotoxin, was used to stimulate podophyllotoxin production in callus and suspension cultures of Juniperus virginiana L. The best phenylalanine effect on podophyllotoxin production was manifested in three-years-old callus cultures after a 21-days application of a 10 mmol/L concentration. A podophyllotoxin content of 0.15 mg/g DW was determined, which was about 400% higher in comparison with the control. The maximum content (0.48 mg/g DW) in newly derived suspension cultures (the 4th passage) was induced by 14-days application of a 1 mmol/L concentration; this was about 243% higher than the control. In one-year-old suspension cultures the highest podophyllotoxin content (0.56 mg/g DW) was recorded also after 14-days application of a 1 mmol/L concentration; this was about 211% higher than in the control cultures.

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Plant Tissue Cultures of Juniperus virginiana

Cited by 3 publications

References 10 publications

Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Effect of Precursor and Phytohormones on Podophyllotoxin Production in Juniperus virginiana Suspension Cultures

Production of Podophyllotoxin by Plant Tissue Cultures of Juniperus virginiana

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