Effect of Precursor and Phytohormones on Podophyllotoxin Production in <i>Juniperus virginiana</i> Suspension Cultures

Kašparová, Markéta; Pilařová, Pavla; Tůmová, Lenka; Siatka, T

doi:10.1177/1934578x1801301129

Cited by 4 publications

(1 citation statement)

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“…The highest maximum podophyllotoxin content was determined at 168 h after the addition of 5 mmol/L MeJA (max. 0.68 mg/g DW) and was about 2.83 times higher than the control sample [ 29 ]. Anbazhagan et al conducted a study on the effect of MeJA elicitation on podophyllotoxin accumulation in suspension and adventitious root cultures of Podophyllum peltatum species.…”

Section: Resultsmentioning

confidence: 99%

Stimulation of Lignan Production in Schisandra rubriflora In Vitro Cultures by Elicitation

et al. 2022

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The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) Schisandra rubriflora Rehd. et Wils. (Schisandraceae) lines. The elicitors were supplemented on the 10th day of culture. Biomasses were collected at 24 h and 48 h, and 4, 6, and 8 days after the addition of each elicitor. The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC–MS/MS method. The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h). In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW). The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures. The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of S. rubriflora emphasize the innovativeness of the research.

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Section: Resultsmentioning

confidence: 99%