Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production

Abstract

The seeds were surface-sterilized with 70% (v/v) ethanol for 45 s and 2.5% (w/v) sodium hypochlorite solution for 12 min, and then rinsed 3 times in sterile distilled water. The seeds were then germinated on MS medium (Murashige and Skoog, 1962) solidified with 8 g/L plant agar, and maintained in a growth chamber at 25 ± 2 °C with a 16-h photoperiod of cool white fluorescent light (400-500 lx).

Extraction, Identification And Determination of Antiviral And Anticancer Flavonoids By HPLC-DAD In Cell Suspension Culture of Melia Azedarach L.

“…In the present study, callus and cell suspension cultures of M. azedarach L. were achieved for the rst time from different explants and PGRs combinations that could be used in biotechnological and molecular studies, and secondary metabolites investigations and production. Oure ndings con rm the previous studies showing that PGRs combination of the medium and explant type and source play a crucial role in plant cell suspension culture establishment and growth (Farjaminezhad et al 2013; Bernabe-Antonio et al 2017; Farjaminezhad and Garoosi, 2019), so the growth of the M. azedarach L. cell cultures were varied depending on the explant type and PGRs. Although the callus induction from petiole explants was high compared to leaf explants, growth rate of the callus and cell suspension cultures derived from the petiole explants were very low as compared to the cell cultures derived from leaf callus.…”

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)

“…High regeneration efficiency with using immature embryo in bread wheat have been reported by Rasco-Gaunt et al [27] and Ozias-Akins and Vasil [28], while Khurana et al [26] reported lower regeneration ability in immature embryos than mature embryos. Plant growth regulators are other important factors affecting callus induction and somatic embryogenesis in plant tissue cultures [3,29]. The auxin type and concentration is the most important factor of medium which determines the somatic embryogenesis in the cereals [9].…”

In-vitro Callogenesis and Screening of Antimicrobial Activity of Callus and Seed of Caesalpinia bonducella F.: A Threatened Medicinal Plant of Western Ghats

“…In recent years, In vitro culture has achieved major industrial importance in the production of secondary metabolites, and callus cultures have been employed in the study of secondary metabolism. The In Vitro Callogenesis technique provides an appealing alternative for the production of valuable secondary metabolites, and they have been used throughout the years as a tool for the elucidation of the biosynthesis of metabolites [15].…”