In vitro regeneration of innervated forelimb deplants of <i>Ambystoma</i> larvae

Liversage, Richard A.; Globus, Morton

doi:10.1139/z77-155

Cited by 6 publications

(1 citation statement)

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“…35 A series of grafting and in vitro studies in the latter half of the 20th century implicated proliferating blastemal cells as a major target of the nerves. In 1977, Liversage & Globus showed that blastemas cultured in vitro proliferated only if they remained innervated by spinal cord implants, 36 while Goldhamer et al found that implantation of dorsal root ganglia into the blastemas of denervated limbs rescued cell cycling, although it did not increase cell cycling in fully-innervated blastemas. 37 Further characterization of cell cycling in the blastema showed that denervation effectively halts mitosis in blastemal cells, 38,39 and thus the modern study of nerve dependence has largely focused on elucidating the links between peripheral nerves and blastemal growth (Fig.…”

Section: History Of Nerve Dependence Research In the Salamandermentioning

confidence: 99%

A brief history of the study of nerve dependent regeneration

Farkas

Monaghan

2017

Neurogenesis

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Nerve dependence is a phenomenon observed across a stunning array of species and tissues. From zebrafish to fetal mice to humans, research across various animal models has shown that nerves are critical for the support of tissue repair and regeneration. Although the study of this phenomenon has persisted for centuries, largely through research conducted in salamanders, the cellular and molecular mechanisms of nerve dependence remain poorly-understood. Here we highlight the near-ubiquity and clinical relevance of vertebrate nerve dependence while providing a timeline of its study and an overview of recent advancements toward understanding the mechanisms behind this process. In presenting a brief history of the research of nerve dependence, we provide both historical and modern context to our recent work on nerve dependent limb regeneration in the Mexican axolotl.

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Section: History Of Nerve Dependence Research In the Salamandermentioning

confidence: 99%

A brief history of the study of nerve dependent regeneration

Farkas

Monaghan

2017

Neurogenesis

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Muscle and cartilage differentiation in axolotl limb regeneration blastema cultures

Hinterberger

Cameron

1983

J. Exp. Zool.

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A tissue culture system is described for explants of mesenchyme from Ambystoma mexicanum limb regeneration blastemas. Explants were cultured on collagen substrate for 3 weeks in minimal essential medium supplemented with the hormones insulin, thyroxine, somatotropin, and hydrocortisone, plus beef embryo extract (EE), 2%. This medium supported extensive cell migration onto the substrate followed by cell proliferation and differentiation of both cartilage matrix and myotubes. Cultures on plastic substrate, rather than on collagen, displayed similar cell outgrowth and cartilage formation, but relatively little myotube formation. Differentiation in EE-supplemented medium was compared with that in two defined media: Explants in medium containing only the hormones showed little outgrowth or cartilage development and never formed myotubes; medium containing the hormones plus fibroblast growth factor, 50 ng/ml, supported an intermediate degree of outgrowth and cartilage development and occasional myotube formation. Explant size was also a factor: Smaller explants survived and formed myotubes less frequently, even when on collagen in EE-supplemented medium.

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In vitro studies of the influence of prolactin on tail regeneration in the adult newtNotophthalmus viridescens

Liversage

Stewart

McLaughlin

1984

Wilhelm Roux' Archiv

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In vitro experiments were carried out to determine the effects of prolactin, and prolactin in combination with other hormones on the regeneration of adult newt tail blastemata. A total of 271 blastemata were explanted 13 days postamputation and were organ cultured for 96 h at 20 (±1)°C. Treatment with prolactin alone resulted in an increase in the blastema cell density of the tail regenerates. Cell accumulation and cell alignment were observed ventral to the reconstituted spinal cord. Prolactin and thyroxine, in combination, improved development of tail regenerates as compared with treatment with prolactin or thyroxine singly, supporting the results of earlier in vivo studies. Optimal development was obtained only when prolactin, insulin, thyroxine and hydrocortisone were added to the culture medium. Regeneration of tail explants maintained in medium augmented with the four hormones closely resembles that of in vivo tail blastemata 17 days post-amputation.

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In vitro regeneration of innervated forelimb deplants of Ambystoma larvae

Cited by 6 publications

References 0 publications

A brief history of the study of nerve dependent regeneration

A brief history of the study of nerve dependent regeneration

Muscle and cartilage differentiation in axolotl limb regeneration blastema cultures

In vitro studies of the influence of prolactin on tail regeneration in the adult newtNotophthalmus viridescens

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