In Vitro Reconstitution of Skin: Fibroblasts Facilitate Keratinocyte Growth and Differentiation on Acellular Reticular Dermis

Krejci, Niels C.; Cuono, Charles B.; Langdon, Robert C.; McGuire, Joseph

doi:10.1111/1523-1747.ep12491522

Cited by 114 publications

(63 citation statements)

References 18 publications

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“…Thus, the functional influence of the secreted cocktail of growth factors and cytokines by the various subpopulations of fibroblasts on keratinocyte proliferation remains unclear. Despite the possible differences between the subpopulations, it is well described that a direct cell-cell contact is crucial to promote keratinocyte growth because fibroblast-conditioned medium cannot substitute for feeder cells [Briggaman and Wheeler, 1968;Krejci et al, 1991].…”

Section: Functional Effects Of Fibroblast Subpopulationsmentioning

confidence: 99%

Diversity of Fibroblasts – A Review on Implications for Skin Tissue Engineering

Nolte

Rennekampff

et al. 2007

Cells Tissues Organs

148

112

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Enormous advances in the development of skin substitutes have occurred in the past 3 decades. Major obstacles yet to be overcome in the quest for an optimal skin substitute include controlling scar formation, contraction and the loss of adnexal structures. Mesenchyme-derived signals are essential for epithelial proliferation, skin morphogenesis, homeostasis and differentiation. Having previously shown that fibroblasts differentiate along a lineage from highly proliferative progenitor fibroblasts with characteristic spindle-shaped appearance to differentiated postmitotic polygonal fibrocytes, we have now established that the different subsets of fibroblasts exert significantly different patterns of cytokine release and that the highest levels of keratinocyte growth factor and transforming growth factor-β₁ expression result from differentiated fibroblasts. Coculture studies with keratinocytes reveal that postmitotic fibroblasts stimulate keratinocyte proliferation to a greater extent than progenitor fibroblasts. Acellular and fibroblast-seeded dermal substitutes have been shown to improve scarring and contraction in animal studies, the latter substitutes yielding the most favorable results. Fibroblasts from different body sites display different functional properties which may affect their suitability for dermal substitutes. Future in vivo human studies in tissue-engineered dermal substitutes will likely focus on fibroblast-seeded lattices and the impact of fibroblast subpopulations and bone marrow-derived mesenchymal stem cells on dermal regeneration.

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Section: Functional Effects Of Fibroblast Subpopulationsmentioning

confidence: 99%

Diversity of Fibroblasts – A Review on Implications for Skin Tissue Engineering

Nolte

Rennekampff

et al. 2007

Cells Tissues Organs

148

112

View full text Add to dashboard Cite

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“…Primary human dermal fibroblasts are the major dermal cell type so their growth should be supported directly on the dermal scaffold [17]. Dermal fibroblasts enhance dermo-epidermal regeneration, stimulate epidermal growth and differentiation and thereby promote wound healing [30][31][32][33][34][35]. We propose that a cell-interactive elastic scaffold would limit wound contraction and thereby minimize scarring and persist in the wound bed long enough for healing to occur.…”

Section: Introductionmentioning

confidence: 99%

Primary human dermal fibroblast interactions with open weave three-dimensional scaffolds prepared from synthetic human elastin

Rnjak

Maitz

et al. 2009

Biomaterials

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“…Several investigations have reported on the use of either AlloDerm or deepidermized human dermis in the devel-opment of reconstituted skin and oral mucosa. [13][14][15][16][17] We reported on the characteristics of an ex vivo-produced oral mucosa equivalent (EVPOME) utilizing AlloDerm in a serum-free, defined medium, without an irradiated xenogeneic feeder layer. 18 In preparation for human clinical trials it was necessary to optimize the culture protocol for the in vivo grafting of EVPOME.…”

Section: Introduction Smentioning

confidence: 99%

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

et al. 2003

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The aim of this study was to determine the optimal stage of development at which transplant human ex vivo-produced oral mucosa equivalents (EVPOMEs) in vivo. EVPOMEs were generated in a serum-free culture system, without the use of an irradiated xenogeneic feeder layer, by seeding human oral keratinocytes onto a human cadaveric dermal equivalent, AlloDerm. EVPOMEs were cultured for 4 days submerged and then for 7 or 14 days at an air-liquid interface to initiate stratification before transplantation into SCID mice. AlloDerm, without epithelium, was used as a control. Mice were killed on days 3, 10, and 21 posttransplantation. Epithelium of the transplanted EVPOMEs was evaluated with the differentiation marker keratin 10/13. Dermal microvessel ingrowth was determined by immunohistochemistry with a mouse vascular marker, lectin binding from Triticum vulgaris. The presence and stratification of the epithelium were correlated with revascularization of the underlying dermis. The microvessel density of AlloDerm without epithelium was less than that of EVPOMEs with an epithelial layer. Microvessel density of the dermis varied directly with the degree of epithelial stratification of the EVPOMEs. The EVPOMEs cultured at an air-liquid interface for 7 days had the optimal balance of neoangiogenesis and epithelial differentiation necessary for in vivo grafting. 163

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In Vitro Reconstitution of Skin: Fibroblasts Facilitate Keratinocyte Growth and Differentiation on Acellular Reticular Dermis

Cited by 114 publications

References 18 publications

Diversity of Fibroblasts – A Review on Implications for Skin Tissue Engineering

Diversity of Fibroblasts – A Review on Implications for Skin Tissue Engineering

Primary human dermal fibroblast interactions with open weave three-dimensional scaffolds prepared from synthetic human elastin

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

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