Primary human dermal fibroblast interactions with open weave three-dimensional scaffolds prepared from synthetic human elastin

Electrospun Tropoelastin for Delivery of Therapeutic Adipose-Derived Stem Cells to Full-Thickness Dermal Wounds

“…1). In agreement with prior studies, 20,21 we observed changes in final scaffold porosity when using different protein solution ejection rates during electrospinning.…”

Elastin Based Constructs

“…Scanning electron micrographs of (A) human umbilical vein endothelial cells, (B) SMCs and (C) dermal fibroblasts cultured on synthetic elastin fibers These types of synthetic elastin hydrogels can be made by chemical cross-linking (Mithieux, Rasko et al 2004), enzyme treatment (Mithieux, Wise et al 2005) or raising the pH (Mithieux, Tu et al 2009) of rhTE solutions (Figure 4). The hydrogels demonstrate mechanical properties that are consistent with native elastin including low elastic moduli, support of attachment and proliferation of dermal fibroblasts (Mithieux, Rasko et al 2004;Rnjak, Li et al 2009;Annabi, Mithieux et al 2010). Increases in hydrogel porosity using high pressure CO 2 or the incorporation of glycosaminoglycans improve cell infiltration into hydrogels (Annabi, Mithieux et al 2010;Tu, Mithieux et al 2010) where the maintenance of fibroblasts within these scaffolds present them as candidate dermal substitutes.…”

“…rhTE exhibits many properties of native tropoelastin including the ability to coacervate under physiological conditions and be cross-linked in vitro to form insoluble elastin fibers (Vrhovski, Jensen et al 1997;Muiznieks, Jensen et al 2003). rhTE promotes endothelial cell and fibroblast attachment, spreading and proliferation when used as a surface coating (Bax, Rodgers et al 2009;Rnjak, Li et al 2009;Wise, Byrom et al 2011) and improves the biocompatibility of implanted devices (Yin, Wise et al 2009;Wilson, Gibson et al 2010). Representative photographs of a synthetic human elastin scaffold (A) before cross-linking and (B-C) after cross-linking with hexamethylene diisocyanate and wetting with phosphate buffered saline.…”

“…Electrospun synthetic elastin is formed by the electrospinning and chemical cross-linking of rhTE to yield ribbon-like microfibers ( Figure 5) whose dimensions match those of native elastin fibers (Nivison-Smith, Rnjak et al 2010). Highly porous electrospun synthetic elastin scaffolds, generated by using high flow rates facilitate the infiltration of dermal fibroblasts in vitro and present an alternative to synthetic elastin hydrogels as a dermal replacement (Rnjak, Li et al 2009). …”

Glyaderm® dermal substitute: Clinical application and long-term results in 55 patients

“…These scaffolds are currently undergoing in vitro and early in vivo testing [44]. In the clinical setting often logistic, financial and temporal issues continue to challenge the burn surgeon to use dermal substitutes on a more larger scale.…”