Electrospun Tropoelastin for Delivery of Therapeutic Adipose-Derived Stem Cells to Full-Thickness Dermal Wounds

Abstract

Objective: To evaluate the physiological effects of electrospun tropoelastin scaffolds as therapeutic adipose-derived stem cell (ADSC) delivery vehicles for the treatment of full-thickness dermal wounds. Approach: Using the process of electrospinning, several prototype microfiber scaffolds were created with tropoelastin. Initial testing of scaffold biocompatibility was performed in vitro through ADSC culture, followed by scanning electron microscopy (SEM) for assessment of ADSC attachment, morphology, and new extracellular matrix (ECM) deposition. The wound healing effects of ADSC-seeded scaffolds were then evaluated in a murine dermal excisional wound model.

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

“…These bioengineering techniques provide the ability to customize the mechanical characteristics, elasticity, and rates of degradation and to guide stem cell lineage differentiation. 84 Matrikines and matri- Figure 5. Novel use of collagen mimetic peptides for sensing and delivery.…”

Blended Polyurethane and Tropoelastin as a Novel Class of Biologically Interactive Elastomer

“…The previous use of electrospun tropoelastin alone and as a copolymer for PCL and collagen has demonstrated its utility in materials engineering for dermal 32 and vascular applications 33 in particular. Screening of electrospinning parameters for E2A identified 100 mg/mL (10% w/v) as the optimal concentration for efficient collection coverage and control of fiber parameters, when using HFP as a solvent.…”

Stem Cell and Stem Cell-Derived Molecular Therapies to Enhance Dermal Wound Healing

“…These features also make electrospun scaffolds an attractive platform for stem cell delivery to chronic wounds. A variety of nanofi brous scaffold have been developed for stem cell delivery, including electrospun tropoelastin [ 135 ], silk fi broin/chitosan [ 73 ], silk fi broin/ poly(lactide-co-glycolic acid) [ 136 ], aloe vera-polycaprolactone (AV/PCL) [ 137 ], poly ( L -lactide-co-ε-caprolactone)/poloxamer [ 138 ], and poly(lactic acid)/silk/ collagen [ 139 ]. Generally, scaffolds seeded with MSCs enhanced wound closure in preclinical wound models, compared to scaffolds alone, or untreated wounds [ 73 , 135 , 137 , 138 ].…”