In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst

In the surgical history of hepatic hydatid disease, multiple scolicidal agents have been used for sterilization of the cyst contents. However, none of these agents can be safely used, because most have unacceptable side effects, such as toxicity, caustic sclerosing cholangitis, bile duct stricture, and hypernatremia. Protoscolices were aseptically removed from liver hydatid cysts obtained from sheep slaughtered at the municipal abattoir in Kahramanmaras, Turkey. In this study, the effects of 0.9% NaCl (control group), 20% NaCl (hypertonic saline), 20% silver nitrate, albendazole 20 mg/cm3, 50% dextrose (hypertonic glucose), and 20% mannitol and aminomix-1 solutions on echinococe cysts were investigated under in vitro conditions. After 5, 10, 15, 20, 25, 30, 45, and 60 min for each concentration, the first 100 protoscolices were counted on the 10x microscopical field. Protoscolices, which showed positive staining by eosine were considered as dead ones. The averages of dead and total protoscolices were calculated. Our results showed that all observed protoscolices were dead after the treatments by 20% silver nitrate in 20 min, by 50% dextrose and by aminomix-1 solution in 30 min, and by 20% NaCl and by 20% mannitol in 45 min. Albendazole at 20 mg/cm3 was observed to lead to death of 65% of protoscolices in the first 5 min and 70% of protoscolices at the end of 60 min. Compared with 0.9% NaCl (saline), all of these scolicidal agents were significantly effective (p < .05). Aminomix-1, 20% mannitol, and 50% dextrose solutions may be used in percutaneous and surgical treatment of hydatid cyst. They may be preferred because they are readily available, can be administered intravenously, and have an equal or greater effectiveness than 20% hypertonic saline.

Section: Discussionmentioning

confidence: 67%

In Vitro Effectiveness of Different Chemical Agents on Scolices of Hydatid Cyst

Çağlar

Yüzbaşıoğlu

Bülbüloğlu

et al. 2008

“…For this reason, scolocidal agents such as hypertonic saline or silver nitrate are often used for the killing of protoscoleces inside the cyst during the surgery. Different protoscolicidal agents such as 70%-95% ethanol, 15%-20% hypertonic saline solution and 0.5% cetrimide solution or even hypertonic glucose have been used both in vivo and in vitro with variety of effects [3,13]. It has been suggested that more experimental studies and clinical observations are urgently needed for evaluating the efficacy and safety of protosclolicides [9].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Screening of DifferentAllium SativumExtracts on Hydatid Cysts Protoscoleces

Sadjjadi

Zoharizadeh

Panjeshahin

2008

Self Cite

This study was conducted to assess the protoscolicidal effect of garlic (Allium sativum) extract on protoscoleces of hydatid cysts in vitro. Viability of protoscoleces was determined, as the percent of viable protoscoleces to the total protoscoleces in 1 ml of the hydatid fluid, using eosin stain method. Four types of garlic extracts including: aquaus extract, chloroform extract (with two concentrations) and hydro-alcoholic extract along with cetrimide and silver nitrate as positive control, and normal saline as negative control, were used in this study. In vitro tests were performed at 37 degrees C with exposure times of 30 minutes, one, two, four, and six hours. The protoscolecidal activity of the used materials was determined by counting of 250 protoscoleces. The mean percent and standard deviation (SD) of protoscolecidal activity of different used materials were as follows: cetrimide: 100 +/- 00, garlic chloroform extract with 200 mg/ml concentration: 99.58 +/- 1.63, garlic chloroform extract with 100 mg/ml concentration: 96.06 +/- 7.28, silver nitrate: 95.91 +/- 7.11, garlic hydro-alcoholic extract: 59.82 +/- 21.48, garlic simple extract: 40.91 +/- 18.1, and of saline: 16.01 +/- 6.25. The protoscolecidal activity increased by the time of exposure. Results of this study showed that chloroform extract of garlic with 200 mg/ml concentration have the highest and simple extract of garlic have the lowest protoscolecidal activity.

“…In vitro evaluation of protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst using 10%, 25%, and 50% glucose solutions showed that protoscolicidal effect of 50% glucose solution was higher than 0.5% silver nitrate and weaker than 0.5% cetrimide, but the effect was comparable with that of 20% hypertonic saline solution [9].…”

Section: Introductionmentioning

confidence: 91%

“…So, in treatment attempts, surgeons utilize several protoscolicidal agents such as cetrimide, silver nitrate, hypertonic saline, and povidone iodine [7,8]. The 0.5% silver nitrate, 0.5% cetrimide, and 20% hypertonic saline solutions are considered as old protoscolicidal agents, but 15% and 25% dextrose solutions are the new ones [9]. The use of silver nitrate is more popular in Iran, but hypertonic saline is still the solution of choice in many countries [7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Different Chemical Agents on the Germinative Layer of Sheep Hydatid Cyst After Implantation to Peritoneal Cavity of Balb/C

Mansourian

Sadjjadi

Hosseini

et al. 2009

Self Cite

In spite of the use of protoscolocidal agents during hydatid cyst surgery, a notable rate of disease recurrence in postoperation patients is still observed. The question remains whether living protoscolices lead to recurrence or the recurrence is due to the remainder of the germinative layer in the peritoneal cavity. The aim of this study was in vivo evaluation of different chemical (protoscolicidal) solutions on the germinative layer of the hydatid cyst. The germinative layer of sheep hydatid cyst was separated under sterile condition, divided into 0.25-cm(2) parts, and exposed to 0.5% cetrimide, 0.5% silver nitrate, 20% hypertonic saline, 15% dextrose and 25% dextrose, and normal saline as negative control for 2 min. The exposed germinative layers were implanted into the peritoneal cavity of 90 Balb/C mice (15 mice in each group). After nine months, the peritoneum was evaluated macroscopically as well as microscopically for the presence of any hydatid cyst. No hydatid cyst was observed in the peritoneal cavity of the exposed mice. The role of the germinative layer for inducing hydatid cysts in mice is questionable. However, the present study showed that the germinative layer had no role in the induction of hydatid cyst in these laboratory animals.