Evaluation of Different Chemical Agents on the Germinative Layer of Sheep Hydatid Cyst After Implantation to Peritoneal Cavity of Balb/C

Abstract

In spite of the use of protoscolocidal agents during hydatid cyst surgery, a notable rate of disease recurrence in postoperation patients is still observed. The question remains whether living protoscolices lead to recurrence or the recurrence is due to the remainder of the germinative layer in the peritoneal cavity. The aim of this study was in vivo evaluation of different chemical (protoscolicidal) solutions on the germinative layer of the hydatid cyst. The germinative layer of sheep hydatid cyst was separated under sterile condition, divided into 0.25-cm(2) parts, and exposed to 0.5% cetrimide, 0.5% silver nitrate, 20% hypertonic saline, 15% dextrose and 25% dextrose, and normal saline as negative control for 2 min. The exposed germinative layers were implanted into the peritoneal cavity of 90 Balb/C mice (15 mice in each group). After nine months, the peritoneum was evaluated macroscopically as well as microscopically for the presence of any hydatid cyst. No hydatid cyst was observed in the peritoneal cavity of the exposed mice. The role of the germinative layer for inducing hydatid cysts in mice is questionable. However, the present study showed that the germinative layer had no role in the induction of hydatid cyst in these laboratory animals.

Solidification of hydatid cyst fluid with an injectable chitosan/carboxymethylcellulose/β-glycerophosphate hydrogel for effective control of spillage during aspiration of hydatid cysts

“…A combination of these treatments can be a fairly effective method in its treatment (Smego et al 2003 ; Smego and Sebanego 2005 ; Yagci et al 2005 ). If human hydatid cyst ruptures due to injury or any other reasons, parasite materials (protoscoleces) spread in the adjacent viscera and any protoscolex can make a potential daughter cyst and possibly anaphylaxis shock or occasionally death (Hosseini et al 2006 ; Mansourian et al 2009 ). Due to the existing problems in the treatments of hydatid cyst, solidification of hydatid cyst fluid can eliminate the risk of daughter cyst formation.…”

KLF4 upregulation is involved in alternative macrophage activation during secondary Echinococcus granulosus infection

“…eggs (oncospheres) by ungulates and humans (intermediate hosts), a larval stage, the metacestode, develops into fluid‐filled cysts in internal organs (mainly liver and lungs), which contain the larva worms or protoscoleces (PSC) (primary infection) . PSC are the infective stage for the definitive host (mainly dogs), but they are also able to reverse differentiation generating new cysts when released in the humans after spontaneous or trauma induced cysts rupture, result in an important medical problem, the human secondary CE (secondary infection) . Secondary infection can be divided into two stages: an early stage in which PSC develop into hydatid cysts (infection establishment) and a later stage in which already differentiated cysts grow and eventually become fertile cysts (chronic infection).…”