In vitro properties of concentrated canine platelets stored in two additive solutions: a comparative study

Abstract

BackgroundPlatelet transfusion therapy poses many challenges in veterinary clinical practice. Lack of readily available blood donors, short shelf-life, and inability to administer a sufficient number of platelets to meet a dog’s transfusion need are the major difficulties encountered. Platelet additive solutions are already in use at American and European human blood banks, showing to be a realistic alternative. This study compares the in vitro platelet function in plasma, Composol, or SSP+ during storage for 13 days. Platelet rich plasma-platelet concentrate with 35% plasma and 65% platelet additive solutions (Composol or SSP+) and a control group (100% plasma) were prepared. Swirling, platelet count, blood gases, metabolic variables, platelet activation markers, and apoptosis markers were analyzed on days 1, 5, 9 and 13.ResultsSwirling was well preserved and pH was acceptable (> 6.2) during storage for all platelet additive solutions units until day 9. SSP + units showed more stable pH and metabolic variables until day 13. Platelets in plasma showed higher glucose consumption than in Composol or in SSP+. The platelet additive solutions units showed better platelet metabolism maintenance, reduced glucose consumption and lactate production. The apoptotic markers were still low for 9 days in platelet concentrates with platelet additive solutions, suggesting the possibility to extend the shelf life with the use of SSP+ or Composol.ConclusionsOur findings suggest that the uses of Composol and SSP+ in canine platelet concentrates are potential alternatives in veterinary blood banks.Electronic supplementary materialThe online version of this article (10.1186/s12917-017-1236-8) contains supplementary material, which is available to authorized users.

Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days

“…In our study, we evaluated stored canine PC on days 1, 3 and 5 of storage with platelet aggregation test, biochemical assays and flow cytometry analysis. Our results were consistent with previous reports from human [12,13] and canine PC studies [1,3,4,14]. Canine PC showed a decrease in pH, glucose, bicarbonate, swirling and pCO 2 values, and an increase in lactate and pO 2 values.…”

“…PC bags were left undisturbed for one hour after preparation, and then were stored at 20-24°C in a platelet environmental chamber (model CDCI 03, Indrel, São Paulo, BRA) under constant stirring (AP48L linear plate agitator, Presvac, BA, ARG). Aliquots were removed for analyses using a sampling site coupler previously sterilized with 70% alchool (Fenwal, Lake Zurich, USA) on days 1, 3, and 5 [4]. All PC were kept out of store for sampling for a maximum of 60 s. On day 5 of storage, all 22 PC were submitted to aerobic and anaerobic microbiological culture in brain heart infusion (BHI) incubated at 37°C for 10 days.…”

“…However, there is limited availability of platelet concentrate (PC) because it is difficult to obtain and has a very short storage period. Although some studies evaluating the quality of canine PC obtained by the platelet-rich plasma (PRP) method under routine blood bank processing and storage have been reported [1][2][3][4], the quality control in veterinary transfusion medicine is still based on human blood bank protocols.…”

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

“…Hoareau et al (2014) [161], compared PRP and buffy coat (BC) protocols for preparation of canine platelet concentrates (PC) and demonstrated that PRP-PC had lesser white blood cells (WBC) and RBC contamination and better platelet functionality than BC-PC. Hlavac et al (2017) [162] demonstrated the effects of storage on different canine platelet concentrates preparations by comparing the in vitro quality maintenance (metabolic evaluation, cell death, mitochondrial membrane polarization, and activation), with data obtained from humans. The authors, demonstrating similar results, suggested the use of canine platelet concentrates as potential alternatives in veterinary blood banks.…”

Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

“…Twenty‐two canine PC were prepared with platelet‐rich plasma method and stored as reported elsewhere . Mitochondrial platelets from canine PC stored in autologous plasma for 5 days were evaluated on days 1, 3, and 5, immediately after sampling.…”