Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

Marcondes, Natália Aydos; Terra, Sílvia Resende; Lasta, Camila Serina; Hlavac, Nicole Regina Capacchi; Dalmolin, Magnus Larruscaim; Lacerda, Luciana de Almeida; Faulhaber, Gustavo Adolpho Moreira; González, Félix Hilario Díaz

doi:10.1002/cyto.a.23567

Cited by 39 publications

(15 citation statements)

References 23 publications

(31 reference statements)

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“…Recently, new applications of this technique have been introduced to monitor physiological and clinical conditions. In the first case, mitochondria viability related to platelet storing process [151][152][153]. For clinical disease, the technique was demonstrated to be useful to detect directly immunoglobulin associated platelets in both healthy and thrombocytopenic dogs [66,67,154].…”

Section: Platelet Laboratory Testing In Dogsmentioning

confidence: 99%

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Cortese

Christopherson

Pelagalli

2020

Animals

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Significant progress has been made in the functional characterization of canine platelets in the last two decades. The role of canine platelets in hemostasis includes their adhesion to the subendothelium, activation, and aggregation, leading to primary clot formation at the site of injury. Studies on canine platelet function and advancements in laboratory testing have improved the diagnosis and understanding of platelet-related disorders as well as the knowledge of the mechanisms behind these diseases. This review focuses on the most recent discoveries in canine platelet structure, function, and disorders; and discusses the efficacy of various tests in the diagnosis of platelet-related disorders. With the relatively recent discovery of angiogenetic and reparative effects of growth factors found in platelets, this review also summarizes the use of canine platelet-rich plasma (PRP) alone or in association with stem cells in regenerative therapy. The characterization of proteomic and lipidomic profiles and development of platelet gene therapy in veterinary species are areas of future study with potential for major therapeutic benefits.

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Section: Platelet Laboratory Testing In Dogsmentioning

confidence: 99%

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Cortese

Christopherson

Pelagalli

2020

Animals

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“…We hypothesized that the morphological changes of mitochondria might lead to ATP production disturbance. ATP levels can be used to monitor the status of mitochondrial function, which is vital for healthy or diseased cells (40). Depletion of ATP synthesis leads to the use of most mitochondrial oxygen to produce ROS, generating an increase in cell lesion (11).…”

Section: Discussionmentioning

confidence: 99%

Molybdenum and Cadmium Co-induce Mitochondrial Quality Control Disorder via FUNDC1-Mediated Mitophagy in Sheep Kidney

Yang

Zhou³

et al. 2022

Front. Vet. Sci.

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Molybdenum (Mo), fundamental trace mineral for animals and plants, but undue Mo damages animal health. Cadmium (Cd) is a toxic heavy metal that exists in the environment. Nevertheless, the mechanism of Mo and Cd on mitochondrial quality control are still indistinct. The objective of this research was to explore the effects of mitophagy on mitochondrial quality control via the FUNDC1-mediated by Mo and Cd in sheep kidney. Forty-eight 2-month-old sheep were stochastically divided into four groups, as shown below: control group, Mo [45 mg/kg body weight (BW)] group, Cd (1 mg/kg BW) group and Mo (45 mg/kg BW)+Cd (1 mg/kg BW) group, with 50 days feed technique. The results showed that Mo or/and Cd attract an unbalance of trace minerals and vacuoles and granular degeneration of renal tubular epithelial cells, and increase the number of mitophagosomes and vacuole-mitochondria and LC3 puncta and MDA and H2O2 contents, and decrease ATP content in the kidney. Moreover, Mo or/and Cd treatment could upregulate the mRNA levels of FUNDC1, LC3A, LC3B, PGAM5, DRP1, FIS1 and MFF, and the protein levels of FUNDC1, p-FUNDC1, LC3II/LC3I, DRP1, MFF and FIS1, downregulate the mRNA levels of MFN1, MFN2, OPA1, PGC-1α, SIRT1, SIRT3, FOXO1 and FOXO3, and the protein levels of MFN1, MFN2, OPA1 and PGC-1α. Notably, variations of above-mentioned factors in Mo and Cd group were more obvious than in Mo or Cd groups. Taken together, these results displayed that Mo and Cd co-treatment might induce mitochondrial quality control disorder via FUNDC1-mediated mitophagy in sheep kidney.

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“…Percentages of platelets positive for CD61 and CD62P were recorded. Assessment of Δψm was performed as described elsewhere [30]. Briefly, platelets were incubated with MitoScreen JC-1 (BD Biosciences, San Jose, USA).…”

Section: Flow Cytometry Analysesmentioning

confidence: 99%

Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days

Lasta

Hlavac

Marcondes³

et al. 2020

BMC Vet Res

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Background: Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytometry and biochemistry assays. Twenty-two bags of canine platelet concentrates were obtained by the platelet-rich plasma method and were assessed on days 1, 3 and 5 after collection. Parameters such as platelet counts, residual leukocytes, platelet swirling, glucose, lactate, pH, CD62P expression (platelet activation), JC-1 (mitochondrial function) and annexin V (apoptosis and cell death) were assessed. Results: Over the five days of storage there was a significant decrease in glucose, HCO 3 , pCO 2 , ATP, pH, swirling and mitochondrial function, associated with a significant increase in lactate levels and pO 2. At the end of storage pH was 5.9 ± 0.6 and lactate levels were 2.8 ± 1.2 mmol/L. Results of the quality parameters evaluated were similar to those reported in human platelets studies. The deleterious effects of storage were more pronounced in bags with higher platelet counts (> 7.49 × 10 10 /unit), suggesting that canine platelet concentrates should not contain an excessive number of platelets. Conclusions: Quality parameters of canine platelets under standard storage conditions were similar to those observed in human platelets. Our results have potential to be used for the routine evaluation and quality control in veterinary blood banks.

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Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

Cited by 39 publications

References 23 publications

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Molybdenum and Cadmium Co-induce Mitochondrial Quality Control Disorder via FUNDC1-Mediated Mitophagy in Sheep Kidney

Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days

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