In vitro Propagation of Vanda testacea (Lindl.) Reichb.f. – A Rare Orchid of High Medicinal Value

Abstract

Foliar explants of Vanda testacea (Lindl.) Reichb. f. were cultured on Mitra (M) medium with 1.0 mg/l BAP, Kn each and 1.0 mg/l NAA individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plantlets. Juvenility of the tissues and chemical stimulus were important factors in initiating the regeneration response in the explants. The relatively older leaf explants (>1cm in length) remained recalcitrant to regeneration the representing younger ones (<1cm in length) responded to certain chemical regimes. BAP, Kn individually in the medium should direct PLB regeneration whereas when used with NAA, the explants showed callus proliferation and further differentiated into PLBs. An individual treatment with NAA (1.0 mg/l) impaired the response frequency and delayed further morphogenetic processes leading to plantlet development. The best response in the explants (in terms of high regeneration frequency, early initiation, PLB proliferations, and plantlet development) was observed in 1.0 mg/l BAP alone/with 1.0 mg/l NAA + activated charcoal. Plantlets were transferred to pots containing epiphytic compost (1 charcoal : 1 brick pices : 1 bats). Nearly 75% of plantlets survival was recorded.

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

“…In the present study, 50 g·dm -3 banana homogenate supported healthy (robust) growth of plantlets. Similar effects was also reported for other orchids -Dendrobium and Spathoglottis kimballiana (Minea et al 2004), Coelogyne flaccida (Kaur & Bhutani 2014) and Dendrobium chrysotoxum initiated from seeds (Kaur & Bhutani 2011). The promotory effect of Bh could be related to high content of carbohydrates in bananas.…”

“…In this condition, use of alternative explants helps in raising innumerable genetically identical clones. So far, the regenerative potential of stemnodes has been positively tested in some orchid species (Arditti & Ernst 1993;George & Ravishankar 1997;Vij & Kaur 1998;Kanjilal et al 1999;Gangaprasad et al 2000;Pyati et al 2002;Decruse et al 2003;Basker & Narmatha Bai 2006;Martin 2007;Zhao et al 2007;Janarthanam & Seshadri 2008;Medina et al 2009;Rangsayatorn 2009;Hong et al 2010;Kaur & Bhutani 2010).Till date, in vitro propagation of D. chrysotoxum was reported only through seeds (immature/mature), shoot-tips and protocorm segments culture (Xu et al 2001;Roy et al 2007;Kaur & Bhutani 2011).The objective of this study was to access the regeneration competence of stemnodes to multiply the D. chrysotoxum by subjecting them to varying physical and chemical stimulus in the medium.…”

Asymbiotic Seed Germination and Synthetic Seed Preparation of Vanda Testacea (Lindl.) RCHB.F

“…Orchids are faced with habitat destruction pressures due to extensive collections in the past. As a consequence the species has become rare and is restricted to very narrow pockets in its natural habitats (Kaur and Bhutani 2009). Thus to conserve this orchid from extinction and to increase the population size, plant tissue culture and micropropagation can play a significant role (Wochok, 1981).…”

Studi Embriogenesis Klon-klon Vanda Hasil Persilangan Vanda tricolor x [(Vanda Patao x Vanda Jenny Hashimoto) x Ascocenda Peggy Foo] secara In Vitro

“…Inkubasi terang dilakukan pada 12 fotoperiode pada suhu yang sama di bawah lampu fluoresen dengan intensitas [13][14][15][16][17][18][19][20][21][22][23][24][25] …”