Asymbiotic Seed Germination and Synthetic Seed Preparation of Vanda Testacea (Lindl.) RCHB.F

Abstract

A protocol for asymbiotic seed germination and synthetic seed preparation of Vanda testacea(Lindl.) Rchb.f. from mature pods was developed. Seed germination was successfully established on half strength B5 (Gamborg's medium) supplemented with 1.0mg/L GA3. Protocorm like bodies (PLB's) were formed after 90 days of culture and further artificial seed preparation of matured protocorm was successful using 5% sodium alginate and 100mM calcium chloride. The beads were inoculated on half strength B5 medium supplemented with 1.0mg/L GA3.