In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture

Utami, Edy Setiti Wida; Hariyanto, Sucipto; Manuhara, Yosephine Sri Wulan

doi:10.1016/j.apjtb.2017.01.011

Cited by 50 publications

(36 citation statements)

References 18 publications

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“…8D) and fresh weight of plantlet (0.7  0.5 g) (Fig. 8F) of high concentration of BH, ie 150 g.L -1 (15%) [35). This shows that regeneration of P. angulata plantlet could be induced with low concentrations of BH.…”

Section: Root Induction and Plantlet Regenerationmentioning

confidence: 71%

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.)

Apensa

Mastuti

2018

JELS

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In vitro culture techniques can be used as an alternative problem solving in an effort to provide Ciplukan plant as a raw material of herbal medicine in a sustainable manner. The purpose of this study was to observe the effect of banana homogenate (BH) added into culture medium on shoot regeneration of Ciplukan (Physalis angulata L.) and plantlet development until acclimatization stage. The experimental design used was a randomized factorial group. The first factor was the two varieties of banana fruit namely, Ambon and Raja. The second factor was concentration of BH with four levels, 0 (control), 2.5%, 5% and 7.5 % and each treatment combination was repeated five times. Explant used in this research was the third node of 2 weeks old in vitro shoot after subculture to MS0 medium. Data analysis used ANOVA test. Eight week after culture (WAC) medium containing BH induced higher number of shoot compare to medium without BH. The addition of 5% BH var. Ambon had significant effect on increasing shoot number. Well rooted plantlets produced in MS0 rooting medium also showed high survival rate (66.7%<-100%) two weeks after transferred to greenhouse. Therefore, the addition of BH into in vitro medium can be an alternative method for micropropagation of Ciplukan.

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Section: Root Induction and Plantlet Regenerationmentioning

confidence: 71%

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.)

Apensa

Mastuti

2018

JELS

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“…Recent studies have shown that coconut water in the culture medium significantly stimulates the formation of roots and shoots in Dendrobium lasianthera J.J.Sm. orchid species (Wida et al, 2017). However, in this research the most efficient culture medium was that supplemented with pineapple juice.…”

Section: Discussionmentioning

confidence: 87%

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Mercado

Contreras

2017

Acta Agron.

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Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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“…Cabe resaltar la importancia de una etapa apropiada en la cual la cápsula debe ser cosechada (Gupta, 2016), de ahí que se hable de cápsulas inmaduras. En las referencias se encuentra que las cápsulas de semilla pueden ser cosechadas a los 75 días después de la polinización cruzada (Suh, 2016) Apertura de Cápsulas y Siembra de Semillas Exposición de la semilla: se sostiene por la base y se realizan cortes longitudinales con bisturí quirúrgico a lo largo de las uniones (Utami et al, 2017). Luego desde uno de los extremos se desprende la superficie de la cápsula sosteniéndola con firmeza de uno de los extremos.…”

Section: Esterilización Superficial De Las Cápsulasunclassified

“…Las orquídeas juegan un papel importante en los sistemas tradicionales de medicina, ya que poseen gran cantidad de sustancias importantes (Bai, 2015;S. N. T. M. N. Banerjee, 2013;Pradhan, Regmi, Ranjit, & Pant, 2016;Pradhan, Tiruwa, Subedee, & Pant, 2014;Utami, Hariyanto, & Manuhara, 2017) tales como flavonoides, triterpenos y saponinas (Mencias & Salazar, 2018). Estas especies sobresalen por su flor, la cual es la parte más atrayente de la planta, y la que más varía en su tamaño, poseen tres sépalos y tres pétalos; de estos dos son iguales y el otro es la parte más llamativa y se denomina labelo; cuya función es la tracción de los insectos polinizadores, el polen es producido en la columna, y en la punta vamos a encontrar la antera; el polen esta empaquetado (entre 4 y 8 paquetes) en masas denominadas polínios (Mencias & Salazar, 2018).…”

Section: Introductionunclassified

Modificaciones en medios de cultivo aplicadas en conservación y producción in-vitro de orquídeas

Salgado¹,

Peñaranda²

2019

recia

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The orquidáceas are a family of plants with economic and scientific interest given their ornamental diversity and applications in medicine among others. However, many species of orchids are threatened and in danger of extension, due to the transformation of the habitat and the excessive extraction of species with commercial interest. Added to this, its propagation and conservation in vitro, is expensive, due to the biological complexity. The present study reports information on a versatile culture medium, focused on the conservation of germplasm and in-vitro production of orchids. To this end, a formulation was designed based on the modification of the Murashige and Skoog (1962) medium with Morel vitamins, coconut water and activated carbon, using the combination of successful experiences from the reported literature. Capsules of Prosthechea fragrans (Sw.) W.E.Higgins were collected in the coffee axis and sterilized in the laboratory. The culture media were tested for callus induction, proliferation of protocormos type structures, development and maintenance of seedlings. Asymmetric germination in-vitro of collected seeds was achieved, three types of callus were obtained. After 7 months of cultivation, the seedlings reached between 1.1. The sterilization protocol of the capsules was validated as well as the protocol of preparation of medium, sowing and subculture, with respect to the results reported in the literature using conventional culture media. The results show that the modifications made in the MS culture medium, allow to reduce the costs of the culture medium by 10% and make it versatile, allowing the micropropagation and maintenance of seedlings of Prosthechea fragrans (Sw.) W.E.Higgins; present in the coffee axis and the establishment of a germplasm bank for the conservation and production of high quality plant material derived from in vitro culture.

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In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture

Cited by 50 publications

References 18 publications

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.)

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.)

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Modificaciones en medios de cultivo aplicadas en conservación y producción in-vitro de orquídeas

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