In vitro propagation of Prunus tenella Batsch. cv. ‘Firehill’: control of vitrification; increase of the multiplication rate and growth by chilling

Bouza, Lydia; Martin, Jacques; Maziere, Y.; Arnaud, Y.

doi:10.1016/0304-4238(92)90016-6

Cited by 11 publications

(11 citation statements)

References 14 publications

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“…The use of the bottom cooling for 3 wk (BC3) affected the proliferation rates in explants of`Lorna'. These results are in agreement with previous researchers (Bouza et al, 1992) who avoided the hyperhydricity by decreasing the RH in the growth room but obtained a decrease in the proliferation rates.…”

Section: Discussionsupporting

confidence: 96%

“…According to Vanderschaege and Debergh (1987), reduction in RH can be achieved by placing the cultures on a cooled plate, which causes the water vapor to condense on the agar medium and this approach allowed hyperhydricity to be controlled. Similar results have been obtained working with Prunus tenella (Bouza et al, 1992): reduction of RH eliminated hyperhydricity in the following passage, but it resulted in a progressive decline in the multiplication rates.…”

Section: Introductionsupporting

confidence: 87%

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Control of hyperhydricity in micropropagated apricot cultivars

Pérez‐Tornero

Egea

Olmos

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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The effects of different factors on the control and reversion of hyperhydricity during the in vitro propagation of Prunus armeniaca were studied. Treatments that decreased the hyperhydricity but did not affect micropropagation rates were the use of the bottom cooling system for 1 or 2 wk and agargel as gelling agent in`Helena', whereas the best results were obtained with the bottom cooling system for 2 wk and the use of 0.8% agar as gelling agent in`Lorna'. Hyperhydric shoots reverted to normal after keeping them for 3 wk in the bottom cooling system.

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Section: Discussionsupporting

confidence: 96%

Section: Introductionsupporting

confidence: 87%

Control of hyperhydricity in micropropagated apricot cultivars

Pérez‐Tornero

Egea

Olmos

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…In this paper, the use of a modified medium and the application of a three-stage procedure drastically improved rooting. An induction period in the dark is often required but it might produce chlorosis and shoot necrosis (Bouza et al, 1992). Both findings were confirmed for cv.…”

Section: In Vitro Rooting and Acclimatizationmentioning

confidence: 80%

Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Koubouris

Vasilakakis

2006

Plant Cell Tiss Organ Cult

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Factors affecting successful establishment in vitro, rapid proliferation and rooting of apricot cultivar 'Bebecou' were studied. Ethanol and NaOCl were applied in several combinations for disinfection; chilling, plant growth regulators BA, IAA and GA 3 , antibiotics, different culture vessels and systems of subculture were evaluated for the optimization of shoot proliferation and the auxins NAA and IBA were assessed for root induction. The highest number of new microshoots/explant (18.7) was obtained in a culture medium supplemented with 2.2 lM BA+0.57 lM IAA after 300 h of chilling. The effect of GA 3 (11.4 lM) on shoot proliferation was positive in combination with 4.4 or 8.9 lM BA. Shoot length and productivity were highest at 2.2 lM BA+11.4 lM GA 3 +0.57 lM IAA and at 2.2 lM BA+0.57 lM IAA, respectively and decreased as cytokinin concentration increased. The antibiotic 'Na-cefotaxime' had a minimal impact on shoot growth when used at the lowest concentration (250 mg l )1 ). Subculture every 2 weeks in a medium supplemented with 2.2 lM BA and 0.57 lM IAA was more efficient for shoot induction than alternation of 20 days culture in a propagation medium supplemented with 2.2 lM BA and 10 days culture in an elongation medium supplemented with 1.

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“…The greater multiplication rate observed in the present study may be mainly related to the genotype and the formulation of the culture medium. The saline formulation developed by Quoirin et al (1977) for in vitro culture of woody fruit trees presents reduced concentration of total nitrogen, reduction of (Bouza et al, 1992;Murai et al, 1997;Pérez-Tornero and Burgos, 2000).…”

Section: Resultsmentioning

confidence: 99%