Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Abstract

Factors affecting successful establishment in vitro, rapid proliferation and rooting of apricot cultivar 'Bebecou' were studied. Ethanol and NaOCl were applied in several combinations for disinfection; chilling, plant growth regulators BA, IAA and GA 3 , antibiotics, different culture vessels and systems of subculture were evaluated for the optimization of shoot proliferation and the auxins NAA and IBA were assessed for root induction. The highest number of new microshoots/explant (18.7) was obtained in a culture medium supplemented with 2.2 lM BA+0.57 lM IAA after 300 h of chilling. The effect of GA 3 (11.4 lM) on shoot proliferation was positive in combination with 4.4 or 8.9 lM BA. Shoot length and productivity were highest at 2.2 lM BA+11.4 lM GA 3 +0.57 lM IAA and at 2.2 lM BA+0.57 lM IAA, respectively and decreased as cytokinin concentration increased. The antibiotic 'Na-cefotaxime' had a minimal impact on shoot growth when used at the lowest concentration (250 mg l )1 ). Subculture every 2 weeks in a medium supplemented with 2.2 lM BA and 0.57 lM IAA was more efficient for shoot induction than alternation of 20 days culture in a propagation medium supplemented with 2.2 lM BA and 10 days culture in an elongation medium supplemented with 1.

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

“…Rooting of the plants was best achieved on Murashige and Skoog (MS) medium supplemented with 2.0 mg L -1 indole-3-butyric acid (IBA) (Yildirim et al, 2011). However, a diff erent pattern of morphogenic response was obtained for the cultivar 'Bebecou', where the combination of 2.2 μM BA and 0.57 μM IAA induced the best micropropagation rate (Koubouris et al, 2006). Especially in apricot, it was previously established that cultivars needed diff erent media compositions to develop efficient shoot formation and plant development (PerezTornero and Burgos, 2000).…”

The Issues of Apricot (Prunus Armeniaca L.) Micropropagation

“…There have been several researches about Prunus armeniaca L. micropropagation which are focused on media compounds (Gago et al, 2011, Jain andHaggman, 2007;Koubouris and Vasilakakis, 2006;Kramarenko, 1999) for instance carbon energy source, effect of hormones (Marino et al, 1991 regeneration from different organs (Escalettes and Dosba, 2003;Peixe et al, 2004;Pérez-Tornero et al, 1999;Pérez-Tornero et al, 2000;Yildirim et al, 2007;Yildirim et al, 2011), hyperhydricty (Pérez-Tornero et al, 2001 and grafting (Errea et al, 2001) as well as many papers focused on virus elimination (Brison et al, 1997;Hauptmanová and Polák, 2011). Plant transfer to aseptic culture and their multiplication for their elimination from viruses were the goals of this research.…”

Effect of Different Hormonal Treatments on Proliferation and Rooting of Three Persian Walnut (Juglans regia L.) Genotypes

“…The culture contamination during in vitro culture should be consider. Seventy percent alcohol, sodium hypochlorite (2.5%), calcium hypochlorite and mercuric chloride (0.01%) for exogenous contaminations and antibiotics recommended to control the inner tissues adulterations (Sticklen and Oraby, 2005;Ainsley et al, 2001;Koubouris and Vasilakakis, 2006;Sharma et al, 1999). Also, nano silver particles suggested to control the bacterial contaminations (Nomiya et al, 2004;Sondi and Salopek-Sondi, 2004).…”