In vitro propagation of Oroxylum indicum Vent. a medicinally important forest tree

Abstract

In vitro propagation of Oroxylum indicum Vent. was carried out using cotyledonary node explants. Among the different types of cytokinins used for culture establishment, 6-benzyladenine exhibited the best response with higher concentrations (8.87 µM or above) for inducing multiple shoots. Inclusion of indole-3-acetic acid (2.85 µM) into 6-benzyladenine-supplemented medium triggered a high frequency of response as well as a proliferation of shoots. The best medium for proliferation was Murashige-Skoog (MS) medium with 6-benzyladenine (8.87 µM) and indole-3-acetic acid (2.85 µM). However, incorporation of gibberellic acid (1.44 µM) was mandatory to enhance shoot elongation. Repeated subculturing of cotyledonary node and in vitro developed nodal segments in MS medium with 6-benzyladenine (4.44 µM) at 4-week intervals resulted in continuous mass multiplication of shoots without any evidence of decline. Root induction was best (91.6%) when MS strength was reduced to one-quarter and combined with α-naphthalene acetic acid (2.69 µM) and indole-3-acetic acid (5.71 µM), with a high survival rate (70-72%) of plantlets hardened in either soil rite or soil : sand : soil rite (1 : 1 : 2).

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

“…In NAA supplemented media, a fair amount of callus was observed at the basal region of the explants. Similar results have also been reported in a number of plant species including Cedrela fissilis [28], Oroxylum indicum [29], and Barleria greenii [30]. The antagonistic influence of auxin for BAP supplemented media for axillary shoot proliferation may be due to exogenous application of auxin.…”

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

“…In the present study, shoot tip and nodal segments obtained from two year old plants were used as explants. Better plant regeneration from node of young trees was also reported in Oroxylum (Dalal and Rai 2004), Boswellia ovalifoliolata (Chandrasekhar et al 2005), Pterocarpus santalinus (Rajeswari and Paliwal 2006), Stereospermum personatum (Shukla et al 2008). Browning of explants due to exudation of phenolics has been reported as a serious problem in establishing axenic cultures of tree species (Krishna and Singh 2007).…”

Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum (L.) Vent

“…Shoot regeneration from embryonic axis explant (both direct regeneration as well as through callus) has been studied previously in O. indicum (Dalal and Rai 2004, Gokhale and Bansal 2005, Gokhale and Bansal 2008.…”