In vitro propagation of chestnut (Castanea sativa×C. crenata): Effects of rooting treatments on plant survival, peroxidase activity and anatomical changes during adventitious root formation

Gonçalves, José Carlos; Diogo, Graça; Amâncio, Sara

doi:10.1016/s0304-4238(97)00136-2

Cited by 63 publications

(78 citation statements)

References 15 publications

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“…Os resultados obtidos neste trabalho foram satisfatórios, uma vez que, para outras espécies, como Malus zumi foi obtido 86,3% de enraizamento ex vitro no melhor tratamento (XU et al, 2008). Comparando a sobrevivência das plantas de Castanea sativa x C. crenata enraizadas in vitro e ex vitro, GONÇALVES et al (1998) A altura das plantas após 30 dias de aclimatização foi maior na cultivar 'Elegance Ice', obtendo-se 6,1cm de altura. Esse valor representa mais de 50% de crescimento das plantas em relação à altura inicial de 4,0cm.…”

Section: Resultsunclassified

Enraizamento de microestacas de Lavandula angustifolia

et al. 2011

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Section: Resultsunclassified

Enraizamento de microestacas de Lavandula angustifolia

et al. 2011

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“…All developed roots, regardless of the medium on which they were induced and the way of formation (with callus and without callus) originated from the division region within the phloem tissue. In in vitro cultivated shoots of Malus, Alnus, Castanea and Cedrela the initial meristemoids of adventitious roots develop either within the phloem adjacent to cambium, at ray parenchyma or cortical and phloem parenchyma (Zhou et al 1992;Goncalves et al 1998;Naija et al 2008;Millán-Orozco et al 2011;San José et al 2012). Mitotic activity is also observed within the core.…”

Section: Discussionmentioning

confidence: 99%

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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The study aimed at evaluating the response of common plum (Prunus domestica L.) microshoots during in vitro rooting in the presence of two phytoactive medium supplements, i.e. a dialyzate of pineapple pulp and a conditioned medium containing green algae Desmodesmus subspicatus exudates. Rooting efficiency was evaluated after 4 weeks of culture. During the root induction phase the content of phenolic compounds in shoot bases was determined and anatomical studies were conducted. Medium supplements were analyzed for the content of carbohydrates and phenolic acids. Both supplements were efficient in rooting induction of shoots of a difficult-to-root cultivar 'Węgierka Zwykła'. Medium supplementation allowed a significant reduction in the exogenous auxin content required for rooting. The highest rooting efficiencies on supplemented media were 28.9 and 27.8 %, in comparison with 33.3 % obtained in the control medium with doubled concentration of exogenous auxins. In the easy-to-root plum cultivar 'Węgierka Dąbrowicka' the rooting rate was slightly reduced in the presence of pineapple dialyzate, while in the presence of algal conditioned medium the rooting rate decreased substantially compared with the nonsupplemented medium. Approximately 30 % of 'Węgierka Dąbrowicka' shoots rooted on supplemented auxin-free media. The content of phenolic compounds accumulated in shoot bases during the root induction stage reflected the differences in rooting ability between both plum cultivars, indicating potential stressful conditions of the culture generated by the presence of phytoactive natural supplements. Anatomical study allowed to recognize the mode of dedifferentiation leading to adventitious rhizogenesis in the common plum. The results are discussed in relation to the composition of medium supplements and their potential root-promoting activity.

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“…Nevertheless, the adventitious roots formed in the control microcuttings (no IBA in the culture medium), were originated always in the pericycle layer, and were similar to the roots of seedlings (Fig.7-B). The excellence of the root system, as mentioned by Gonçalves et al (1998), is a key factor for the success of the acclimatization process. The incomplete vascular connection between shoot and roots of cauliflower rooted in vitro resulted in insufficient water translocation to the shoot, endangering the acclimatization of the new plants (Grout & Aston, 1977).…”

Section: Resultsmentioning

confidence: 99%