In vitro propagation of Campanula glomerata, ‘Acaulis’ from leaf blade explants

Joung, Young Hee; Liao, Mingjun; Roh, Mark S.; Kamo, Kathryn; Song, Jaekyeong

doi:10.1016/s0304-4238(01)00282-5

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…It is typically propagated by division of the shoots or by seeds. Shortage of healthy seeds and plant material has often been affecting the mass propagation of this important horticultural species (Joung et al 2002). Large scale production of elite clones through micropropagation or somatic embryogenesis solves this problem.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, somatic embryogenesis is useful for artificial seed production (Maruyama et al 2003), cryopreservation (Yin and Hong 2010;Kong and von Aderkas 2011), genetic manipulations (Capelo et al 2010;Khan et al 2010;Parimalan et al 2010), and large scale propagation (Chen et al 2010;Menéndez-Yuffá et al 2010). A limited number of studies have been focused on in vitro propagation of Campanula species (Sriskandarajah et al 2001;Frello et al 2002;Joung et al 2002;Sivanesan et al 2007). However, till date there is no report described about somatic embryogenesis in Campanula species.…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

Sivanesan

Lim

Jeong

2011

Plant Cell Tiss Organ Cult

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A simple and efficient protocol was developed for somatic embryogenesis from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino. Somatic embryos (SE) were obtained with greater frequency from petiole explants than from leaf explants when cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L -1 6-benzyladenine (BA). On this medium, a mean number of 19.5 and 31.2 SE were developed per leaf and petiole explants, respectively. Embryos were induced both light and dark conditions but culturing the explants 2 weeks in the dark followed by 3 weeks under light resulted in high frequency of embryo formation. Globular embryos germinated best on MS medium supplemented with 0.3% (w/v) activated charcoal (AC) and 1.0 mg L -1 GA 3 . The germinated plantlets grew further on MS medium containing 0.3% AC. Plantlets were successfully acclimatized in the greenhouse with 94% survival rate. This is the first report on induction of somatic embryogenesis in this genus and also has implications for genetic transformation, and mass clonal propagation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

Sivanesan

Lim

Jeong

2011

Plant Cell Tiss Organ Cult

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“…Regeneration systems for Campanula carpatica and Campanula glomerata have been established (Sriskandarajah et al 2001;Frello et al 2002;Joung et al 2002), and a method for transforming C. glomerata containing the uidA gene and the nptII gene was also reported recently (Joung et al 2001). After establishing regeneration protocols for C. carpatica, we continued our studies to establish a transformation system.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: factors affecting transformation and regeneration of transgenic shoots

Sriskandarajah¹,

Frello²,

Jørgensen³

et al. 2004

Plant Cell Rep

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An efficient transformation system for Campanula carpatica was developed using Agrobacterium tumefaciens strains LBA4404 (harbouring the plasmid pBI121), and AGL0 (harbouring the plasmid pBEO210). This is the first report on the transformation of C. carpatica. Various factors affecting the transformation efficiency and subsequent regeneration were identified. The age of seedlings from which the explants for transformation studies were taken, and the growth conditions under which the seedlings were grown had a significant influence on the production of transformed shoots. Hypocotyls taken from 12-day-old seedlings grown in the dark were the most productive, with up to 25% of hypocotyls producing transformed shoots. Explants taken from 5-week-old seedlings produced only transformed callus. The medium used for co-cultivation and incubation also had a significant influence on transformation frequency and shoot regeneration. The cultivar "Blue Uniform" was more responsive than "White Uniform". Both bacterial strains and plasmids were equally effective in producing transformed tissue. Transformed shoots were selected on kanamycin medium, and the presence of the uidA and nptII genes in those selected shoots was confirmed by beta-glucuronidase and ELISA analyses, respectively.

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“…Basal medium used was MS, supplemented with 30 g l 21 sucrose, 6 g l 21 agar, and pH 5.8 (Brandt 1992(Brandt , 1994Joung et al 2002).…”

Section: Effect Of Cytokininsmentioning

confidence: 99%

“…However, these methods are time consuming and require increased number of mother plants initially. In vitro propagation meets the need to supply sufficient healthy propagation material, in a short period of time (Joung et al 2002). This method has been used for some Campanula species (Brandt 1992(Brandt , 1994Joung et al 2002;Sriskandarajah et al 2001), but variations even among genotypes have been reported (Brandt 1994).…”

Section: Introductionmentioning

confidence: 99%

GIS-facilitatedex situconservation of the rare Greek endemicCampanula incurvaAucher: Seed germination requirements and effect of growth regulators onin vitroproliferation and rooting

Grigoriadou¹,

Krigas

Maloupa³

2014

Plant Biosystems - An International Journal Dealing with all As

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In vitro propagation of Campanula glomerata, ‘Acaulis’ from leaf blade explants

Cited by 14 publications

References 10 publications

Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: factors affecting transformation and regeneration of transgenic shoots

GIS-facilitatedex situconservation of the rare Greek endemicCampanula incurvaAucher: Seed germination requirements and effect of growth regulators onin vitroproliferation and rooting

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