Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

Abstract

A simple and efficient protocol was developed for somatic embryogenesis from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino. Somatic embryos (SE) were obtained with greater frequency from petiole explants than from leaf explants when cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L -1 6-benzyladenine (BA). On this medium, a mean number of 19.5 and 31.2 SE were developed per leaf and petiole explants, respectively. Embryos were induced both light and dark conditions but culturing the explants 2 weeks in the dark followed by 3 weeks under light resulted in high frequency of embryo formation. Globular embryos germinated best on MS medium supplemented with 0.3% (w/v) activated charcoal (AC) and 1.0 mg L -1 GA 3 . The germinated plantlets grew further on MS medium containing 0.3% AC. Plantlets were successfully acclimatized in the greenhouse with 94% survival rate. This is the first report on induction of somatic embryogenesis in this genus and also has implications for genetic transformation, and mass clonal propagation.

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

“…In this study, direct adventitious shoot organogenesis and plant regeneration were first established in N. cadamba using cotyledons as explants. These results were similar to those of previous studies on yam (Anike et al 2012), Solanum aculeatissimum (Ghimire et al 2012), olive (Brhadda et al 2003), Chrysanthemum morifolium (Song et al 2011), Campanula punctata (Sivanesan et al 2011), and Pulsatilla koreana (Lin et al 2011), in which the petioles of the cotyledons or leaves retained sufficient cellular plasticity to achieve plantlet regeneration or somatic embryogenesis. However, a number of parameters are known to influence shoot regeneration.…”

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

“…Azul (Rodríguez-Sahagún et al 2011) and Campanula punctata var. rubriflora (Sivanesan et al 2011) and the induction of protocorm-like bodies in in vitro cultures of orchids -Dendrobium officinale (Lin et al 2011) and Oncidium sp. (Mengxi et al 2011) represent good examples.…”

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

“…In vitro propagation through somatic embryogenesis has become an essential tool for the improvement of various systems (Cardosa et al 2012;Sivanesan et al 2011;Nuno-Ayala et al 2012;Ratanasanobon & Seaton 2010;Baskaran & van Staden 2012). An important application of somatic embryos is that they can be utilized for the preparation of artificial seeds or synthetic seeds which are considered as analogous to the natural seeds.…”