Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Huang, Hao; Li, JunCheng; Ouyang, Kang; Zhao, Xiaoling; Li, Pei; Liao, Boyong; Chen, XiaoYang

doi:10.5511/plantbiotechnology.14.0125a

Cited by 25 publications

(27 citation statements)

References 27 publications

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“…However, we found that BA concentrations higher than 2.0 mg · L -1 in combination with IAA had a negative effect on C. oleifera direct adventitious shoot formation (Table 1). This result was similar to those of previous studies on pumpkin ash (Huang et al, 2014;Stevens and Pijut, 2012). Therefore, the concentration of appropriate PGR was important for early establishment of shoot regeneration.…”

Section: Resultssupporting

confidence: 91%

“…IBA used alone had a better effect on root formation than NAA, but root length and number of lateral roots were decreased (Fig. 2C), which is consistent with previous studies (Huang et al, 2014;Patil et al, 2011;Stevens and Pijut, 2012). NAA also led to the formation of a callus at the base of shoots with low rooting rate, making it difficult for acclimatization and transplantation (Fig.…”

Section: Resultssupporting

confidence: 91%

“…A successful early cotyledonary nodes adventitious organogenesis had also been reported in lentil (Bermejo et al, 2012). In addition, several studies demonstrated that the emergence of adventitious shoots from the cotyledonary petioles was simple (Huang et al, 2014;Song et al, 2011;Sujatha et al, 2012). Such adventitious shoots were restored from epidermal cells with high mitotic activity possibly because epidermal cells of cotyledonary petiole may retain the characteristics of parenchyma cells.…”

Section: Resultsmentioning

confidence: 69%

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In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

Liu

Lin

et al. 2016

horts

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Camellia oleifera Abel. is one of four major woody oil plants in the world. The objective of the current study was to evaluate the effect of different plant growth regulators (PGRs) and concentrations on direct organogenesis using cotyledonary nodes, hypocotyls, and radicle explants. High induction frequency of adventitious shoots were obtained from cotyledonary nodes, hypocotyls, and radicle explants (85.2%, 73.6%, and 41.0%, respectively) when cultured on half-strength Murashige and Skoog (1/2 MS) medium containing 2.0 mg·L−1 6-benzylaminopurine (BA) and 0.1 mg·L−1 indole-3-acetic acid (IAA). Microshoots from cotyledonary nodes, hypocotyls, and radicle explants were then transferred to 1/2 MS medium containing 2.0 mg·L−1 BA and 0.05 mg·L−1 indole-3-butyric acid (IBA) for shoot multiplication, resulting in 6.9 shoots per explant. The shoots were transferred to Woody Plant Medium (WPM) supplemented with various α-naphthalene acetic acid (NAA) and gibberellic acid (GA3) for shoot elongation. The mean length of shoots and the number of leaves per shoot were 3.7 and 6.6 cm, respectively, in WPM supplemented with 0.5 mg·L−1 NAA and 3.0 mg·L−1 GA3. The highest rooting of shoots (90.2%) or the number of roots per shoot (7.2) was obtained when elongated microshoots were transferred to 1/2 MS medium supplemented with 3.5% perlite, 1.0 mg·L−1 IBA and 2.0 mg·L−1 NAA. The rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90.0%. The in vitro plant regeneration procedure described in this study is beneficial for mass propagation and improvement of C. oleifera through genetic engineering.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 69%

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In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

Liu

Lin

et al. 2016

horts

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“…Procedures follow Huang et al 21 . Briefly, elongated adventitious shoots (3-4 cm) were transferred to MS agar medium supplemented with 0.25 μM IBA and 0.27 μM NAA, and cultured at 25 ± 2 °C, first in dark for 2 days and then under a 12-h photoperiod (90 μmol m −2 s −1 ) for 8 days.…”

Section: Root Induction and Plantlet Acclimatizationmentioning

confidence: 99%

High frequency regeneration of plants via callus-mediated organogenesis from cotyledon and hypocotyl cultures in a multipurpose tropical tree (Neolamarkia Cadamba)

Huang

Wei

Zhai

et al. 2020

Sci Rep

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In this works, a simple, efficient and repeatable protocol was developed for in vitro regeneration via callus-mediated organogenesis of Neolamarkia Cadamba using cotyledonary petioles and hypocotyls. Effects of basal medium, plant growth regulators, the types and age of explant on the formation of adventitious buds/shoots were studied. Meanwhile, histological analysis for early ontogenic stages and genetic stability assessment by flow cytometry were investigated. Our investigation demonstrated that, compared with 6-benzyladenine (BA), N 6-(2-isopentenyl) adenine (2-ip), Thidiazuron (TDZ) was the optimal cytokinin for buds/shoots induction on cotyledon and hypocotyl explants. Douglas-fir and sugar pine medium (DCR) supplemented with 22.7 μM TDZ and 0.27 μM α-naphthalene acetic acid (NAA) was most effective on bud induction, with the highest bud-induction rate and numbers of buds on cotyledon and hypocotyl explants. The available shoot per explant hit 35.2 when the induced callus sub-cultured to a medium without TDZ. It was found that TDZ could promote induction of the callus and the buds, however, continuous exposure beyond 4 weeks of supplemented high concentration (exceed 11.35 μM), TDZ was harmful to the proliferation and growth of buds/shoots. DCR appeared more efficiency than Murashige and Skoog medium (MS), Woody Plant medium (WPM), anther culture of cereal crops medium (n 6) on bud induction. Age of cotyledon and hypocotyl explants in 20-day to 25day was most beneficial to adventitious buds/shoots formation. Histological investigation confirmed that the buds originated from the wounded incisions of cotyledonary petiole and hypocotyl fragments, with callus formation. The regeneration plantlets were successfully acclimatized in greenhouse, yielded above 95% survival rate in field, exhibited normal morphology and growth characteristics. The analysis of flow cytometry on N. cadamba indicated no variation in the ploidy levels between the regenerated plantlets and the donor trees. The developed procedure can be used for mass production, germplasm exchange and transgenic studies to improve the resistance of the species via Agrobacterium-mediated. Neolamarckia cadamba (commonly known as burflower tree, kadamba tree or kadamb) is a tropical tree of great economic importance. First, N. cadamba trees grow fast, and their wood is good for pulp production, furniture, and other construction purposes 1. Second, N. cadamba has been traditionally used as medicine to treat diseases such as dysentery, fever and snake bites 2,3. Now, from its bark, leaves, and flowers people extract phytochemical compounds, including monoterpenoid, triterpenoid saponin, and ethylene glycol 4-9 , alkaloid 10 , cadambine 7,11 , and use them to treat hyperglycemia 12 , hyperlipidemia 13,14 , hypertension 15 , and wound and skin diseases 16. Third,

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“…For woody plants, using a cotyledonary node as an explant is relatively easy to promote adventitious shoots (Huang et al, 2014;San-Jos e et al, 2001). Therefore, adventitious shoots were restored from epidermal cells that may retain the characteristics of parenchymal cells.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Chinquapin (Castanea henryi) Using Axillary Shoots and Cotyledonary Nodes

Xiong¹,

Sun²,

Feng³

et al. 2018

horts

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Castanea henryi is an important woody grain tree species native to China. The objective of the current study was to find the suitable plant growth regulators (PGRs) and the optimal concentrations for direct organogenesis by using axillary shoots and cotyledonary nodes. Seeds were collected from the field, sterilized, and germinated in vitro. Axillary shoots and cotyledonary nodes of 3-week-old seedlings were used as explants. To find the suitable PGR for adventitious shoot induction, 0.5 mg · L -1 6-benzylaminopurine (6-BA), 0.1 mg · L -1 indole-3-acetic acid (IAA), 0.1 mg · L -1 2,4-dichlorophenoxyacetic acid (2,4-D), or 0.1 mg · L -1 1-naphthaleneacetic acid (NAA) was supplemented to Murashige and Skoog (MS) medium containing 0.65% agar and 3% sucrose. A high induction percentage of adventitious shoots (85.67%) was obtained from cotyledonary nodes supplemented with 0.1 mg · L -1 2,4-D. The type of explant influenced shoot proliferation rates and quality. Apical explants produced more and longer shoots than nodal segments. For shoot multiplication, 1 mg · L -1 6-BA + 0.05 mg · L -1 indole-3-butyric acid (IBA) supplemented with MS medium produced 12.33 and 6.25 shoots per explant, respectively, from apical and nodal explants. For shoot elongation and strengthening, 2 mg · L -1 6-BA + 0.05 mg · L -1 IBA supplemented with MS medium was the best combination, producing shoots with a mean length of 3.50 cm, a diameter of 0.46 cm, and about eight leaves per shoot. The greatest rooting of 76.70% and 11.33 roots per shoot was achieved when cultured in MS medium supplemented with 3.5% perlite + 1.5 mg · L -1 IBA. For acclimatization of the rooted plantlets in the greenhouse, a survival rate of 80% was achieved. This protocol-from multiplication to acclimation-is helpful to realize mass propagation of high-quality trees of chinquapin for increasing production and nut quality.Chinquapin (Castanea henryi) is an economically important species of the Castanea genus that produces timber and starch, which are widely distributed in southern China, especially in the Fujian, Zhejiang, and Hunan

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Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Cited by 25 publications

References 27 publications

In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

High frequency regeneration of plants via callus-mediated organogenesis from cotyledon and hypocotyl cultures in a multipurpose tropical tree (Neolamarkia Cadamba)

Micropropagation of Chinquapin (Castanea henryi) Using Axillary Shoots and Cotyledonary Nodes

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