GIS-facilitatedex situconservation of the rare Greek endemicCampanula incurvaAucher: Seed germination requirements and effect of growth regulators onin vitroproliferation and rooting

Campanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia (both İzmir and Aydın). This species belongs to the Mediterranean basin floristic region. According to the International Union for Conservation of Nature’s criteria, C. phitosiana is classified as “Critically Endangered” (CR). To develop in vitro regeneration protocol for critically endangered endemic Campanula phitosiana. To investigate the efficient medium and plant growth regulator combinations for callus initiation and shoot proliferation, petiole and leaves were used as explant and explants cultivated on MS medium including NAA (1-Naphthylacetic acid) (0.3 mg L-1), TDZ (Thidiazuron) (0.5, 1.0, 2.0, and 3.0 mg L-1), BA (6-Benzylaminopurine) (0.5, 1.0, 2.0, and 3.0 mg L-1) and Gibberellic Acid (GA3) (0.5, 1.0, 2.0, and 3.0 mg L-1). Explants subcultured 3 times. Experiments were conducted according to completely randomized design repeated with 5 replicates and each replicates including 5 explants. As a result of the experiment, callus initiation and shoot proliferation were investigated. Efficient callus initiation was observed petiole explants as 100% rate. Shoot proliferation was observed on MS medium including 0.3 mg L-1 NAA + 2.0 mg L-1 GA3.

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GIS-facilitatedex situconservation of the rare Greek endemicCampanula incurvaAucher: Seed germination requirements and effect of growth regulators onin vitroproliferation and rooting

Cited by 5 publications

References 27 publications

In vitro propagation of medicinal and aromatic plants: the case of selected Greek species with conservation priority

In vitro propagation of medicinal and aromatic plants: the case of selected Greek species with conservation priority

Sustainable use of mediterranean medicinal-aromatic plants

Investigation of In vitro Propagation Possibilities of Endemic Campanula phitosiana Yıldırım & Şentürk

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