In vitro propagation of Amaryllis belladonna

Bruyn, M. H. de; Ferreira, D. I.; Slabbert, M.M.; Pretorius, J.C.

doi:10.1007/bf00036221

Cited by 26 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results have been reported for various ornamental bulbs (De Bruyn et al 1992;Pierik et al 1990), when split bulblets grown in vitro were used as explants, and for N. bowdenii (Jacobs et al 1992) and Narcissus (Hussey 1982), when propagated by twin-scale explants. Similar results have been reported for various ornamental bulbs (De Bruyn et al 1992;Pierik et al 1990), when split bulblets grown in vitro were used as explants, and for N. bowdenii (Jacobs et al 1992) and Narcissus (Hussey 1982), when propagated by twin-scale explants.…”

Section: Resultssupporting

confidence: 84%

“…Newly developed buds were recognized by one peg-like leaf primordium with procambium strands and a meristematic bulge (Fig. Unlike nerine, in other bulbs, such as A. belladonna (De Bruyn et al 1992), C. macowanii (Slabbert et al 1993), Hippeastrum hybridum (Okubo et al 1990) and Narcissus (Hussey 1982), only one meristematic bulge or protuberance appears after 14, 10-12, 7 or 20 days of incubation, respectively. Procambium strands of the leaf primordium started to develop towards the abaxial side of the inner scale of the halved bulblet.…”

Section: Bulblet Originmentioning

confidence: 99%

“…Different plant parts and organs have been used as explants for nerine micropropagation: scales, twin-scales, ovaries, flower stalk sections and the pedicel/scape junction from the flower stalk (Custers and Bergervoet 1992;Grootaarts et al 1981;Lilien-Kipnis et al 1992;Mochtak 1989;Pierik and Ippel 1977;Pierik and Steegmans 1986;Ziv and Lilien-Kipnis 2000). Although several bulbous plants have been propagated through the splitting of in-vitro-grown bulblets, the highest number formed in vitro in Amaryllis belladonna (De Bruyn et al 1992) or in Hippeastrum hybrids (Pierik et al 1990) was only four bulblets per explant. Although several bulbous plants have been propagated through the splitting of in-vitro-grown bulblets, the highest number formed in vitro in Amaryllis belladonna (De Bruyn et al 1992) or in Hippeastrum hybrids (Pierik et al 1990) was only four bulblets per explant.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro

2003

View full text Add to dashboard Cite

Nerine (Nerine sarniensis) cv. Salmon Supreme in vitro-grown bulblets, 7-9 mm in diameter, were cut in half longitudinally and used for adventitious bud initiation following dissection of the roots and two-thirds of the upper part of the bulblets. The terminal apex was injured with a hot, sterile microscope dissecting needle. The highest number of buds formed (seven to nine buds per halved bulblet) on a semi-solid Murashige and Skoog (MS) basal salts medium supplemented with 3% sucrose and either 1 mM 6-benzylaminopurine (BA) and 1 mM anaphthaleneacetic acid (NAA) or 0.5 mM BA and 0.1 mM NAA. Bulblet halves were cultured in the dark for 11-13 weeks with one subculture after 6 weeks. Anatomical studies indicated that the initiation of adventitious buds on the abaxial side of the inner scales of the halved bulblet was adjacent to the basal plate and started from the leaf primordium and a meristematic bulge. Buds developed directly into small bulblets after they were transferred to semi-solid MS basal salts medium supplemented with 6% sucrose, 10 mM indole-3-butyric acid (IBA) and 0.25% activated charcoal. Small bulblets cultured in liquid MS medium supplemented with additional KH 2 PO 4 (170 mg l -1 ), 6% sucrose and 0.1 mM NAA under a 16/8-h (light/dark) photoperiod for 8 weeks grew into larger bulbs faster than those cultured on semi-solid medium. The bulbs were rooted on a semi-solid medium after 4 weeks and then transferred to the soil. As many as 18 bulblets developed and rooted from one in vitro-grown bulb after 25-27 weeks.

show abstract

Section: Resultssupporting

confidence: 84%

Section: Bulblet Originmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro

2003

View full text Add to dashboard Cite

show abstract

“…Overall, sucrose at 3% (w/v) resulted in higher multiplication of shoots than glucose at all concentrations tested, however, differences were not significant. A number of studies advocate the use of 3% sucrose in culture media (Yaseen et al 2012) and similar results have been reported in Amaryllis belladonna (De-Bruyn 1992), Eclipta alba (Baskaran and Jayabalan 2005), and Quercus suber (Romano et al 1995).…”

Section: Discussionmentioning

confidence: 53%

In vitro propagation of cherry birch (Betula lentaL.)

Rathwell¹,

Shukla²,

Jones³

et al. 2016

Can. J. Plant Sci.

View full text Add to dashboard Cite

An efficient procedure for the micropropagation and conservation of cherry birch (Betula lenta L.), an endangered species in Canada, is reported. The model utilizes in vitro proliferation of fresh and dormant buds from both greenhouse and mature trees. Various factors were evaluated to optimize the protocol, including plant growth regulators, type and concentration of carbohydrates, and the composition of basal salts. 6-benzylaminopurine was the most effective cytokinin for shoot multiplication, with an optimal concentration of 5 μM. Sucrose was a more effective carbohydrate source than glucose, with the highest multiplication rate occurring at 3% sucrose (w/v). Shoot multiplication was similar on Murashige and Skoog (MS) and Driver and Kuniyuki Walnut (DKW) basal salts, but significantly lower on Woody Plant medium (WPM). Culture media with half-strength DKW basal salts and 20 μM indole-3-butyric acid was found to be the best for rooting (80%). Rooted plantlets were acclimatized to the greenhouse environment with a 37% survival rate. The micropropagation technology developed in the study offers excellent opportunities and tools for rapid replenishment of cherry birch trees in their natural environment, long-term conservation, and provides a platform for further research of this nationally endangered species.Key words: micropropagation, Betula lenta, cherry birch, conservation.Résumé : Les auteurs présentent une méthode efficace pour la micropropagation et la préservation du bouleau flexible (Betula lenta L.), une espèce menacée d'extinction au Canada. Le modèle fait appel à la multiplication in vitro de bourgeons frais et dormants prélevés sur des arbres cultivés en serre et matures. Plusieurs facteurs ont été évalués de manière à optimiser le protocole, notamment les hormones de croissance végétales, la nature et la concentration des hydrates de carbone, ainsi que la composition des sels de base. La 6-benzylaminopurine est la meilleure cytokinine pour multiplier les pousses et sa concentration optimale est de 5 μM. Le sucrose est plus efficace que le glucose comme source d'hydrates de carbone, le taux de multiplication le plus élevé ayant été enregistré à la concentration de 3 % (p/v). Les sels de base Murashige and Skoog (MS) et Driver and Kuniyuki Walnut (DKW) permettent une multiplication similaire des pousses, mais celle-ci est significativement plus faible quand on utilise le milieu de culture Woody Plant (WPM). Le milieu de culture contenant la moitié de la dose recommandée de DKW et 20 μM d'acide indole-3-butyrique est le meilleur pour l'enracinement (80 %). Trente-sept pour cent des plantules enracinées ont survécu en serre après acclimatation. La technique de la micropropagation élaborée dans le cadre de cette étude présente d'excellentes possibilités et offre des outils intéressants pour rétablir rapidement la population de bouleaux flexibles dans leur milieu naturel, pour la conservation à long terme et pour des recherches plus poussées sur cette espèce en voie de disparition au pays. [Tra...

show abstract

“…Additionally, they maintained that the endogenous contamination was the greatest problem with the propagation of the tubers. Thermotherapy and fungicides in combination with immersions in NaOCl solutions can lower survival rates and eliminate contamination (Bruyn et al, 1992). …”

mentioning

confidence: 99%

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

et al. 2014

View full text Add to dashboard Cite

K. Paredes, C. Delaveau, P. Carrasco, C. Baeza, Freddy Mora, and M.E. Uribe. 2014. In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Cien. Inv. Agr. 41(2): 207-214. Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant, respectively. The results showed high contamination of the bulbs. There were no significant differences between the treatments with plant growth regulators. A multiplication rate of 1.3 -2.2 for bulbils was achieved, with an average of 28 bulbils per mother bulb. The natural regeneration rate is approximately 1 -4 bulbils per mother bulb once a year. T. modesta is a plant characterized by its small size. It measures 15-20 cm in height, with linear or slightly swollen leaves, which are dry or non present at the time of blooming. Flowers measure 15-20 mm in length and are pure white or white with purple veins near towards to the base, with a purple line along the back of the tepals. The fruits are capsules that contain black-shiny seeds that are flattened, rounded and with membranous edges. In its natural habitat in Chile, T. modesta blooms from January to May (Baeza et al., 2009).Studies related to vegetative propagation of T. modesta are unknown; however, it is known that geophytes from Amaryllidaceae have a low natural propagation rate that ranges from approximately zero to eight bulbs per mother bulb (Santos et al., 1998; Schiappacasse et al., 2002;Angulo et al., 2003). Conventional propagation techniques for ornamental purposes have been used for the recovery of other species of endangered bulbous plants. For example, Herbertia lahue (Mol.) Goldbl. is a species from which Morales et al. (2007) obtained 1.13 bulbils per bulb, which is within the expected range found for other Chilean geophyte species such as Calydorea xiphioides Poepp. and Leucocoryne coquimbensis F. Phil (Kim et al., 1998), which produced 1.5 and 1.1 to 1.6 bulbils per mother bulb, respectively (Morales et al., 2007).In studies performed keep carried out by Schiappacasse et al. (2002), 18 species of Chilean geophytes with ornamental potential were propagated. In these studies, a maximum of 8.6 bulbils per initial bulb were obtained by using bulb division as the method for vegetative propagation. When performing the twin-scaling separation technique with Rhodophiala bagnoldii (Herb.) Traub, the number increased to 30 bulbils per mother bulb, demonstrating the potential of these techniques.To compare the karyotype of T. modesta with a species of the same family, Baeza et al. (2009) conduct...

show abstract

In vitro propagation of Amaryllis belladonna

Cited by 26 publications

References 11 publications

Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro

Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro

In vitro propagation of cherry birch (Betula lentaL.)

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

Contact Info

Product

Resources

About