In vitro propagation of cherry birch (<i>Betula lenta</i>L.)

Rathwell, Ricki; Shukla, Mukund R.; Jones, Andrew Maxwell Phineas; Saxena, Praveen K.

doi:10.1139/cjps-2015-0331

Cited by 19 publications

(26 citation statements)

References 30 publications

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“…Agab & Greuter and Isoplexis isabiliana (Webb & Berth) Masf. [18,19,[32][33][34]. Stunted shoots at high concentrations of BA have also been observed in other plant species [35].…”

Section: Site Type Name Overwinter Survival (%) Flowering (%) Shoot Rmentioning

confidence: 95%

“…prevent species loss in the field. Earlier we developed micropropagation methods for several threatened North American plant species including American elm, cherry birch, and golden paintbrush [18,19,24,25]. The current study referred to as the CPR model was undertaken to assess the potential of micropropagated plants in establishing new plant populations in a range of diverse natural habitats.…”

Section: Plos Onementioning

confidence: 99%

“…Micropropagation is emerging as an efficient tool that has been successfully used as a source of healthy plant material for reintroductions globally [15][16][17]. In Canada, micropropagation has also been applied to propagate plants for in vitro conservation of endangered plant species such as Betula lenta L. and Castilleja levisecta Greenm [18,19]. Micropropagation is a good approach for Hill's thistle because germplasm can be stored to conserve the limited genetic diversity in sterile conditions while the threats to the extant populations are managed through reintroduction of micropropagated plants.…”

Section: Introductionmentioning

confidence: 99%

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Saving threatened plant species: Reintroduction of Hill’s thistle (Cirsium hillii. (Canby) Fernald) to its natural habitat

et al. 2020

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Hill's thistle (Cirsium hillii (Canby) Fernald) is a perennial plant endemic to the Great Lakes region of North America. Hill's thistle is listed as threatened in Ontario and Canada where it is found in globally rare alvar habitats. The main objective of this study was ex-situ conservation of Hill's thistle using in vitro culture techniques and reintroduction of micropropagated plants back to their natural habitat in Bruce Peninsula National Park, Ontario, Canada. Two out of twenty-nine available seeds were successfully germinated under in vitro condition. An efficient micropropagation protocol was optimized with 100% survival during acclimatization of plantlets in the greenhouse. Three hundred micropropagated plants were reintroduced to twelve different sites within Bruce Peninsula National Park in June and July 2017. Plants were monitored for survival, rosette growth, and flowering on all sites from 2017-2019. After four months of planting, 67 to 99% of the plants were alive in different sites and 90 to 99% of them survived over winter. In the following years, shoot regeneration and flowering were observed on most sites. This study further confirms the benefit of plant tissue culture techniques to ensure revival of Hill's thistle ecological biodiversity through the reintroduction of micropropagated plants. This approach consisting of the components of conservation, propagation, and reintroduction (CPR) may potentially serve as a model for saving and enriching other species at risk.

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“…Agab & Greuter and Isoplexis isabiliana (Webb & Berth) Masf. [18,19,[32][33][34]. Stunted shoots at high concentrations of BA have also been observed in other plant species [35].…”

Section: Site Type Name Overwinter Survival (%) Flowering (%) Shoot Rmentioning

confidence: 95%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Saving threatened plant species: Reintroduction of Hill’s thistle (Cirsium hillii. (Canby) Fernald) to its natural habitat

et al. 2020

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“…Cultivation on MS medium has not been so successful (Ditmar 1991, Iliev et al 2003. On the contrary, Rathwell et al (2016) reported more promising results in Betula lenta cultures on MS and Driver and Kuniyuki Walnut medium (DKW -Driver & Kuniyuki 1984) as compared to WP medium. In our case, the shoots were vigorous and green on the control WP medium without BAP supply, but they grew less intensively, reaching just 2 and 4 mm after 4 and 8 weeks, respectively (Tab.…”

Section: In Vitro Culturementioning

confidence: 99%

“…Similarly, Máchová et al (2012) reported 98% success in ex vitro transfer in the case of B. nana. Rathwell et al (2016) reported that 110…”

Section: Iforest -Biogeosciences and Forestrymentioning

confidence: 99%

Conservation of Betula oycoviensis, an endangered rare taxon, using vegetative propagation methods

Vítámvás¹,

Kuneš²,

Viehmannová³

et al. 2020

iForest

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Biogeosciences and Forestry Biogeosciences and Forestry Conservation of Betula oycoviensis, an endangered rare taxon, using vegetative propagation methods Jan Vítámvás (1) , Ivan Kuneš (1) , Iva Viehmannová (2) , Rostislav Linda (1) , Martin Baláš (1) Ojcow birch (Betula oycoviensis Besser) is a rare Central European tree taxon, micro-populations of which are found in only several localities. With a view to maintaining the B. oycoviensis gene pool, this study tested the species' potential for micropropagation, grafting, and propagation by cuttings. Plant material for vegetative propagation was collected from ten genotypes in the Czech Republic. In vitro culture was established from axillary buds surfaces sterilized with 0.1% HgCl2 and cultivated on woody plant (WP) medium supplemented with 1 mg l-1 6-benzylaminopurine (BAP). Two genotypes of the species were successfully multiplied by in vitro propagation using WP medium supplemented with 0-2 mg l-1 BAP. The BAP concentration of 1 mg l-1 proved to be optimal, yielding 2.5 new shoots per explant in genotype 516 and 3.5 shoots per explant in genotype 545. The shoots were rooted on half-strength Murashige and Skoog (MS) medium supplemented with various concentrations of α-naphthylacetic acid (NAA) and indole-3-butyric acid (IBA). The highest rooting percentages (72.5% and 77.5% for genotypes 516 and 545, respectively) were achieved on the medium with the combination of both auxins at concentrations of 0.3 mg l-1. The rooted plants were transferred ex vitro in substrate composed of sand, peat, and perlite (1:1:1) and acclimated in the greenhouse. After 4 weeks, more than 90% of plants survived. Grafting was carried out in spring using Betula pendula as rootstock. The efficiency of this technique ranged from 0% to 50% across genotypes, and 4 out of 10 genotypes were successfully propagated by grafting. The cuttings were treated with commercial root stimulators Stimulax I and Stimulator AS-1, planted in a mixture of peat and sand (1:1) in the greenhouse, and watered regularly. This technique resulted in 0% rooting, however, and no cutting survived until the end of the vegetation period. The results of this study show that protocols for in vitro propagation and grafting can be employed for effective mass propagation of B. oycoviensis, although these processes show genotype-dependent responses.

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Improved in vitro rooting in liquid culture using a two piece scaffold system

Shukla

Piunno

Saxena

et al. 2019

Engineering in Life Sciences

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Plant tissue culture techniques have been used to propagate horticultural crops at a commercial scale for more than three decades. However, due to the high cost it is generally only used for high value crops. To increase production efficiency and make micropropagation viable for a wider range of species, new approaches to address key steps of the process with high labor inputs need to be evaluated. For this study, a two‐piece scaffold system was designed, prototyped using 3D printing, and tested to physically hold plants upright thereby facilitating liquid based rooting. This system was evaluated with Malus domestica, Betula lenta, and Musa sp. using static liquid culture as well as rocker based temporary immersion system and compared to rooting in semi‐solid based medium as is commonly practiced. Significantly, earlier rooting was observed in all three species in liquid when compared to semi‐solid culture system, and plants cultured in liquid on the rocker generally performed better than those in static liquid. In addition to quicker, more uniform rooting, reducing labor requirements, and preventing root damage. This newly designed system is simple, easy to use, will help to improve efficiency, and reduce the cost of micropropagation.

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In vitro propagation of cherry birch (Betula lentaL.)

Cited by 19 publications

References 30 publications

Saving threatened plant species: Reintroduction of Hill’s thistle (Cirsium hillii. (Canby) Fernald) to its natural habitat

Saving threatened plant species: Reintroduction of Hill’s thistle (Cirsium hillii. (Canby) Fernald) to its natural habitat

Conservation of Betula oycoviensis, an endangered rare taxon, using vegetative propagation methods

Improved in vitro rooting in liquid culture using a two piece scaffold system

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