In vitro propagation, ex vitro rooting and leaf micromorphology of Bauhinia racemosa Lam.: a leguminous tree with medicinal values

Abstract

A micropropagation system for Lam. was developed involving axillary shoot proliferation and ex vitro rooting using nodal explants obtained from mature tree. MS medium with 3.0 mg l BA (6-benzyladenine) was optimum for shoot bud induction. For shoot multiplication, mother explants were transferred repeatedly on medium containing low concentration of BA (0.75 mg l). Number of shoots was increased up to two passages and decreased thereafter. Shoot multiplication was further enhanced on MS medium containing 0.25 mg l each of BA and Kin (Kinetin) with 0.1 mg l of NAA (α-naphthalene acetic acid). Addition of 0.004 mg l TDZ (thidiazuron) increased the rate of shoot multiplication and 21.81 ± 1.26 shoots per culture vessel were obtained. In vitro regenerated shoots were rooted under ex vitro conditions treated with 400 mg l IBA (indole-3-butyric acid) for 7 min on sterile soilrite. After successful hardening in greenhouse, ex vitro rooted plants were transferred to the field conditions with ≈85% of survival rate. Micromorphological changes were observed on leaf surface i.e. development of vein density and trichomes and stomatal appearance, when plants were subjected to environmental conditions. This is the first report on in vitro regeneration of from mature tree.

Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT

“…The results generated in the study corroborate the true to type nature of micropropagated plants and authenticate that B. racemosa in vitro cultures developed according to the described protocol (Sharma et al 2017) are free from somaclonal variations. They also support the view that development of in vitro cultures through organized primordia (pre-existing meristems) especially apical meristems and axillary buds is a reliable method of producing genetically stable plant population as the tissue culture of organized meristems circumvent the dedifferentiation and/or redifferentiation of cells or tissues (Negi and Saxena 2010;Phulwaria et al 2013).…”

“…Plant material and extraction of genomic DNA Juvenile leaves were collected from nine plants of B. racemosa randomly selected from the population of micropropagated plants established by using protocol developed by Sharma et al (2017) (Fig. 1a-c).…”

“…No alternative means for the propagation have been developed yet. Therefore, a micropropagation system involving axillary shoot proliferation through mature tree derived nodal explants in B. racemosa was developed by our group (Sharma et al 2017) recently. In continuation of the developed protocol, genetic stability of micropropagated plants developed by Sharma et al (2017) was evaluated using two gene targeted markers i.e.…”

Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

“…However, in vitro rooting considerably increases the cost and time of obtaining micropropagated plants. The ex vitro rooting technique (simultaneous rooting and acclimatization of unrooted microshoots) improves micropropagation efficiency due to time and cost reduction and a simpler process; it also eliminates the risk of root damage during transplantation of rooted plants [30,31]. For instance, ex vitro rooting reduced the cost of micropropagated tea plants by 71% compared with conventional in vitro rooting [32].…”

Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

“…Acclimatization is an important stage, especially in plants resulting from in vitro culture, because in general, the roots of plantlets derived from in vitro culture have different structures so that the ability at the time of adaptation during acclimatization is not the same (Chandra et al 2010). The percentage of successful acclimatization is usually very low, so the treatment and environmental conditions determine the success of acclimatization (Sharma 2017). To adjust to the environmental conditions acclimatization carried out in a way, plantlets were cultured on ion free for a week later after the plantlet was able to remove the new roots in which the plantlet was transferred to the mud media.…”